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目的了解清远市6岁以下病毒性腹泻儿童中的轮状病毒、人杯状病毒、腺病毒及星状病毒4种病毒感染的情况。方法收集2010年10月-2013年9月清远市区医院住院腹泻患儿粪便,采用ELISA法对轮状病毒进行检测,用PCR/RT-PCR对人杯状病毒、腺病毒、星状病毒进行检测,并对腹泻患儿的临床资料进行分析。结果共收集237份标本,轮状病毒、人杯状病毒、腺病毒、星状病毒的阳性率分别为29.1%、32.5%、4.6%、0;混合感染率为5.1%。结论本地区小儿病毒性腹泻的主要病原是人杯状病毒和轮状病毒,秋冬季为发病高峰,7月~2岁儿童为发病高危人群,存在不同病毒混合感染。
Objective To understand the infection of four viruses, rotavirus, human calicivirus, adenovirus and astrovirus among children with viral diarrhea under 6 years old in Qingyuan City. Methods Collecting stool samples of diarrhea children from Qingyuan downtown hospital from October 2010 to September 2013. Rotavirus was detected by ELISA and human calicivirus, adenovirus and astrovirus were detected by PCR / RT-PCR Test, and analysis of clinical data of children with diarrhea. Results A total of 237 specimens were collected. The positive rates of rotavirus, human calicivirus, adenovirus and astrovirus were 29.1%, 32.5%, 4.6%, respectively. The mixed infection rate was 5.1%. Conclusions The main pathogen of pediatric viral diarrhea in the region is human calicivirus and rotavirus. The peak is found in autumn and winter, while the children in July ~ 2 years old are at high risk of developing infection with different viruses.