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[Object]To investigate the optimum pH for seed germination and seedling growth of Glycyrrhiza uralensis,and optimize its industrialization technology.[Methods]The seeds of Glycyrrhiza uralensis were soaked into solutions with different pH for 7 d(pH 3,5,6,7,8,9,10,11),and the germination rate,germination energy,germination index,the length of radicle and plumule,fresh weight of seedlings were determined;the seedlings of Glycyrrhiza uralensis were cultivated in solutions with different pH(5,6,7,8,9) for 30 d,and the biomass,contents of medicinal compositions,protein,amino acids,soluble total sugar,starch and MDA,the activities of nitrate reductase and peroxidase etc.indexes were determined.[Result]The germination rate of Glycyrrhiza uralensis seeds,the length of radicle and plumule all achieved the highest level when pH was 6,and the seedlings came out evenly;besides,the biomass,the contents of medicinal compositions,protein,amino acids,soluble total sugar,starch and MDA as well as the activities of nitrate reductase and peroxidase all reached the maximum value with pH of 6.[Conclusion]The optimum pH for the germination of Glycyrrhiza uralensis seed,the growth of seedlings and the accumulation of medicinal ingredients was 6.
[Object] To investigate the optimum pH for seed germination and seedling growth of Glycyrrhiza uralensis, and optimize its industrialization technology. [Methods] The seeds of Glycyrrhiza uralensis were soaked into solutions with different pH for 7 d (pH 3,5,6, 7,8,9,10,11), and the germination rate, germination energy, germination index, the length of radicle and plumule, fresh weight of seedlings were determined; the seedlings of Glycyrrhiza uralensis were cultivated in solutions with different pH (5 , 6,7,8,9) for 30 d, and the biomass, contents of medicinal compositions, protein, amino acids, soluble total sugar, starch and MDA, the activities of nitrate reductase and peroxidase etc.indexes were determined. [Result ] The germination rate of Glycyrrhiza uralensis seeds, the length of radicle and plumule all achieved the highest level when pH was 6, and the seedlings came out evenly; besides, the biomass, the contents of medicinal compositions, protein, amino acids, soluble total sugar, starch and MDA as well as the activities of nitrate reductase and peroxidase all reached the maximum value with pH of 6. [Conclusion] The optimum pH for the germination of Glycyrrhiza uralensis seed, the growth of seedlings and the accumulation of medicinal ingredients was 6.