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目的 :为观察慢性乙型肝炎患者HBeAg和HBsAgS/N测定值在原血清与稀释血清所占的比例 ;如何使双份血清S/N测定值具有可比性 ;HBeAg、HBsAg滴度与转阴的关系。方法 :将慢性乙型肝炎患者血清从 1 0 0 — 1 0 - 5稀释检测HBeAg ;从 1 0 0 — 1 0 - 9稀释检测HBsAg。双份血清对比在同等条件下检测。结果 :HBeAgS/N值在 1 0 0 者占 4 .1 % ,在 1 0 - 1 — 1 0 - 5者占95 .9% ;HBsAgS/N值在 1 0 0 占 1 3 .4% ,在 1 0 - 1 — 1 0 - 9者占 86 .6 %。 2 8份血清HBeAgS/N值在 1 0 0 者转阴率为 85 .7% ;72份血清S/N值在 1 0 - 1 — 1 0 - 5者转阴率为 5 .6 % ,P <0 .0 0 5。31份血清HBsAgS/N值在 1 0 0 者转阴率为 38.7% ,86份血清HBsAgS/N值在 1 0 - 1 — 1 0 - 9者转阴率为零 (P <0 .0 0 5)。结论 :原血清检测HBeAg、HBsAgS/N值只表示有无感染 ,不能衡量患者血清内病毒抗原量的多少 ;HBeAg、HBsAgS/N值在 1 0 0 者转阴率明显高于 1 0 - 1 以上者 ;双份血清在同等条件下的不同滴度S/N值对比是考核HBeAg、HBsAg量变的较理想的指标
OBJECTIVE: To investigate the ratio of HBeAg and HBsAgS / N in serum and diluted serum in patients with chronic hepatitis B, how to make double serum S / N ratio comparable, and the relationship between HBeAg and HBsAg titer and negative conversion . Methods: HBeAg was detected by dilution of serum from patients with chronic hepatitis B from 100-10, and HBsAg was detected by dilution from 100-10. Double serum test under the same conditions. Results: HBeAgS / N value was 4.1% in 100 cases, 95.9% in 100-1-10-5 cases, and HBsAgS / N value was 130.3% in 100 cases. 1 0 - 1 - 1 0 - 9 accounted for 86.6%. The HBeAgS / N value of 28 serum samples was lower than 100% at 85.7%; 72 serum S / N values at 10-1-1 0-5 were 5.6%, P <0. 0 0 5.31 serum HBsAg / N value of 100% negative conversion rate of 38.7%, 86 serum HBsAgS / N value of 10 -1 -1 0-9 negative conversion rate of zero ( P <0 0 05). CONCLUSION: The detection of HBeAg and HBsAg / N value in the original serum only indicates the presence or absence of infection and can not measure the amount of viral antigen in the serum. The negative conversion rate of HBeAg and HBsAg / ; Double serum in different conditions under the same titer of different titer S / N ratio is to assess HBeAg, HBsAg quantitative indicators of the more ideal