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利用RNA-seq鉴定抗寒苹果矮化砧木‘71-3-150’冷胁迫(4°C)响应过程中的转录因子。结果表明:该砧木冷胁迫2、6、12和24 h时的样品与未处理相比,差异表达的转录因子基因数分别为83、98、115和136,共计228个,表明大量转录因子参与该砧木的冷胁迫响应。这些转录因子基因属于32个家族,占苹果转录因子总数的7.25%,包括32个WRKY、30个AP2/ERF、28个b HLH、21个MYB、13个NAC、12个HSF、10个CO-like、9个MYB_related、8个TCP、6个b ZIP、6个C2H2、6个C3H、6个GATA、5个G2-like、4个GRAS、4个HD-ZIP、4个NF-YC、4个ARF、3个Dof、3个Trihelix、2个GRF和2个NF-YB,以及B3、DBB、E2F/DP、EIL、MIKC、M-type、SBP、TALE、YABBY和ZF-HD家族的各1个成员,其中有8个在苹果参考基因组没有注释。这些基因总体上分为10类表达模式,同一类表达模式中的基因均涉及到多个转录因子家族,表明转录调控在该砧木应答冷胁迫中存在复杂机制。根据对鉴定到转录因子基因的功能分析,它们参与了冷适应相关功能基因和生长发育相关功能基因的表达调控,使该砧木在冷胁迫下重建体内物质代谢和氧化还原平衡,获得抗寒性,并且重建适应冷胁迫的新的生长与发育平衡。
Identification of transcription factors during cold stress (4 ° C) response to cold-resistant apple dwarfing stock ’71-3-150 ’using RNA-seq. The results showed that compared with untreated samples, the number of differentially expressed transcription factors was 83, 98, 115 and 136, respectively, for a total of 228 samples at 2, 6, 12 and 24 h, indicating that a large number of transcription factors involved Cold stress response of the rootstock. These transcription factor genes belong to 32 families accounting for 7.25% of the total number of apple transcription factors including 32 WRKYs, 30 AP2 / ERFs, 28 b HLHs, 21 MYBs, 13 NACs, 12 HSFs, 10 CO- like 9 MYB_related 8 TCP 6 b ZIP 6 C2H2 6 C3H 6 GATA 5 G2-like 4 GRAS 4 HD-ZIP 4 NF-YC 4 ARFs, 3 Dofs, 3 Trihelixs, 2 GRFs and 2 NF-YBs, as well as each of the B3, DBB, E2F / DP, EIL, MIKC, M-type, SBP, TALE, YABBY and ZF-HD families One member, eight of which are not annotated in the apple reference genome. These genes are generally divided into 10 types of expression patterns. Genes in the same type of expression pattern all involve multiple families of transcription factors, indicating that transcriptional regulation has a complex mechanism in response to cold stress in the rootstock. According to the functional analysis of the identified transcription factor genes, they are involved in the expression regulation of functional genes related to cold-adaptation and growth-related genes, so that the rootstock can reconstruct the balance of substance metabolism and redox balance under cold stress, And reconstruct a new balance of growth and development that accommodates cold stress.