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应用二对亲本间多态性均高达73%的杂交组合(特三矮2号/CB和外引2号/CB),分别建立二个籼型F2遗传群体,在此基础上,分别构建二张涉及12条染色体的籼稻RFLP连锁图谱。在特三矮2号/CB的图谱上,定位了89个标记位点,总图距为14104cM,标记位点间平均图距为183cM;外引2号/CB的图谱上有93个标记位点,总图距为13285cM,平均为164cM。二张图谱具有较高的相似性,且对水稻基因组覆盖率高,标记位点不存在明显的偏态分离,同时在图谱上分布较均匀且致密,是开展水稻重要农艺性状基因定位尤其是QTL定位的理想图谱。
Two indica F2 genetic populations were established using two crosses (Te3ziao2 / CB and Cited2 / CB) with the polymorphisms of both parents as high as 73% Based on this, two RFLP linkage maps of indica rice with 12 chromosomes were constructed respectively. In the map of Tejiangzai 2 / CB, 89 marker sites were mapped with a total map distance of 14104cM and the average distance between marker sites was 183cM; There are 93 marker sites on the map of B The total map distance is 13285cM, with an average of 164cM. The two maps were highly similar and had high coverage of rice genome. There was no obvious skewed segregation of the marker loci. At the same time, the distribution of the two maps was more uniform and dense, which was the result of QTL mapping of important agronomic traits in rice Ideal positioning of the map.