论文部分内容阅读
目的：：建立测定伤科跌打膏中姜黄素和盐酸小檗碱含量的方法。方法：采用高效液相色谱法。姜黄素：色谱柱：In-ertSutain C18(250 mm ×4.6 mm,5μm)；流动相：乙腈-4%醋酸溶液(44∶56)；流速：1.0 ml·min-1；柱温：25℃；检测波长：430 nm；进样量：10μl。盐酸小檗碱：色谱柱：InertSutain C18(250 mm ×4.6 mm,5μm)；流动相：乙腈-0.1%磷酸溶液(44∶56)(每100 ml加十二烷基磺酸钠0.1 g)；流速：1.0 ml·min-1；柱温：25℃；检测波长：345 nm；进样量：10μl。结果：姜黄素和盐酸小檗碱分别在0.01~0.50μg(r=0.9993)和0.02~0.26μg(r=0.9999)范围内各自呈现良好的线性关系,平均加样回收率分别为为101.03%和99.20%,RSD分别为1.75%和0.64%(n=9)。结论：本研究建立的方法操作简单,准确灵敏,专属性强,可用于伤科跌打膏的质量控制。“,”Objective: To establish a method for the determination of curcumine and berberine hydrochloride in Shangke Dieda paste. Methods:An HPLC method was adopted to determine the content of curcumine and berberine hydrochloride in Shangke Dieda paste. For curcumine, the column was InertSutain C18 (250 mm × 4. 6 mm,5 μm); the mobile phase was acetonitrile and 4% acetic acid solution (44 ∶56);the flow rate was 1. 0 ml·min-1;the column temperature was 25℃;the detection wavelength was 430 nm;the sample size was 10μl. For berberine hydrochloride, the column was InertSutain C18 (250 mm × 4. 6 mm,5μm);the mobile phase consisted of acetonitrile-0. 1% phosphoric acid (44 ∶56, 0. 2 g dodecyl sodium sulfate was added to 100 ml solution); the flow rate was 1. 0 ml·min-1;the column temperature was 25℃ ;the detection wavelength was 345 nm;the sample size was 10 μl. Results:A good linear correlation was obtained within the range of 0.01-0.50 μg (r =0.999 3) for curcumin and 0.02-0.16 μg(r =0. 999 9) for berberine hydrochloride. The average recovery was 101. 03% with RSD of 1. 75% for curcumin and 99. 20% with RSD of 0. 64% for berberine hydrochloride (n=9). Conclusion:The established method is simple, accurate, sensitive and specific, which can be used for the quality control of Shangke Dieda paste.