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本题研究是经免疫学途径直接检测人体感染结核菌的情况，为现代结核病的实验诊断、临床监测、流行病调查提供了一个全新的检验指标。作者首先发现了一种仅存在于活动性结核病患者体液中的蛋白成份—活动性结核标志物（ＡｃｔｉｖｅＴｕｂｅｒｃｕｌｏｓｉｓＭａｒｋ—ＡＴＭ）１Ｈ—多肽；并为之创立了独特的检测方法，经四年多临床１９４６０例样本调查中确定了ＡＴＭ的临床价值。将ＡＴＭ检测与ＯＴ皮试、酶联免疫ＥＬＩＳＡ、ＤＮＡ探针、ＰＣＲ基因扩增技术及典型病例组患者行Ｘ线计算机断层摄影（ＣＴ）、磁共振像（ＭＲＩ）等多组对比试验中，实验与临床研究资料分析证明：ＡＴＭ检测的总敏感度为８６．０６％、特异度９６．２４％、准确度９３．４５％、诊断效率为８２．８２％、批内ＣＶ１．２％、批间ＣＶ２．０％、Ｐ＜０．０５。经ＮＭＲ光谱分析结构含有ＣＣＨ２官能团。
This study is a direct test of human immunodeficiency virus infection of Mycobacterium tuberculosis, for the modern tuberculosis experimental diagnosis, clinical monitoring, epidemiological survey provides a new test indicators. The authors first discovered an active ingredient (ActiveTuberculosisMark-ATM) 1H-polypeptide, which is found in body fluids of patients with active tuberculosis, and created a unique test for this. After more than four years of clinical practice, 19460 patients The sample survey identified the clinical value of ATM. In the comparative experiments of X-ray computed tomography (CT) and magnetic resonance imaging (MRI) between ATM test and OT skin test, enzyme-linked immunosorbent ELISA, DNA probe, PCR gene amplification and typical cases, Experimental and clinical research data analysis showed that the overall sensitivity of ATM detection was 86.06%, specificity was 96.24%, accuracy was 93.45%, diagnostic efficiency was 82.82%, CV1.2% within batch, batch CV2.0% between, P <0.05. The structure of the structure contains CCH2 functional groups by NMR spectroscopy.