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目的在体外培养条件下观察阿伦磷酸钠对大鼠骨髓基质干细胞成骨分化的影响。方法采用四甲基偶氮唑盐(MTT)法检测阿伦磷酸钠对骨髓基质干细胞活性的影响;茜素红染色法来确定骨髓基质干细胞的成骨分化程度;碱性磷酸酶(ALP)活性检测;分光光度法来测定钙峰值,和免疫荧光流式细胞术测定CD44的表达。结果0.1μg/ml、1μg/ml、10μg/ml的阿伦磷酸钠对大鼠骨髓基质干细胞的细胞毒性不明显,都可以促进其增值和成骨分化,浓度为1μg/ml时作用最大,浓度为10μg/ml时作用明显降低。结论适当浓度的阿伦磷酸钠可以促进大鼠骨髓基质干细胞的增值和成骨分化。
Objective To observe the effect of alendronate on the osteogenic differentiation of rat bone marrow stromal stem cells in vitro. Methods MTT assay was used to detect the effect of alendronate on the activity of bone marrow stromal cells. Alizarin red staining was used to determine the osteogenic differentiation of bone marrow stromal cells. The activity of alkaline phosphatase (ALP) Detection; spectrophotometric determination of calcium peak, and immunofluorescence flow cytometry CD44 expression. Results The cytotoxicity of alendronate at 0.1μg / ml, 1μg / ml and 10μg / ml on rat bone marrow stromal stem cells was not obvious, which could promote the proliferation and differentiation of rat bone marrow stromal cells. When the concentration was 1μg / ml, The effect was significantly reduced at 10 μg / ml. Conclusion Allergy sodium phosphate can promote the proliferation and osteogenic differentiation of rat bone marrow stromal stem cells.