8-(N,N-Diethylamino)-n-octyl-3,4,5-trimethoxybenzoate reduced (Ca~(2+))_i elevation induced by

• 作 者: 王斌 张孝清 刘天培 肖继皋
• 机 构: 南京医科大学药理教研室
• 出 处: Acta Pharmacologica Sinica
• 发表日期: 1998年03期
• 关键词: basilar glutamate 基底动脉 色胺 细胞内游离钙 cultured serotonin elevation 平滑肌细胞 octyl

目的：研究８（Ｎ，Ｎ二乙胺）ｎ辛基３，４，５三甲氧基苯甲酸酯对培养乳牛基底动脉平滑肌［Ｃａ２＋］ｉ的作用．方法：采用ＡＲＣＭＭＩＣ阳离子测定系统，测量细胞内游离钙浓度（［Ｃａ２＋］ｉ）．结果：在细胞外钙浓度为１３ｍｍｏｌ·Ｌ－１时，ＴＭＢ８３０μｍｏｌ·Ｌ－１可明显抑制组胺，５羟色胺和谷氨酸引起的［Ｃａ２＋］ｉ的升高．在外钙为零＋依他酸０１ｍｍｏｌ·Ｌ－１时，ＴＭＢ８３０μｍｏｌ·Ｌ－１可明显降低静息［Ｃａ２＋］ｉ，ＴＭＢ８３０μｍｏｌ·Ｌ－１可几乎完全阻断组胺和５羟色胺增加［Ｃａ２＋］ｉ的作用．结论：ＴＭＢ８降低培养乳牛基底动脉平滑肌静息［Ｃａ２＋］ｉ，抑制Ｈｉｓ，５ＨＴ和Ｇｌｕ引起的［Ｃａ２＋］ｉ的增加．

Objective: To study the effect of 8 (N, Ndiethylamine) noctyl  3, 4, 5 - trimethoxybenzoate on cultured [Ca2 +] i in basilar artery of dairy cow. Methods: AR  CM  MCIC cation measurement system, measurement of intracellular free calcium concentration ([Ca2] i). Results: When extracellular calcium concentration was 13mmol·L-1, TMB830μmol·L-1 significantly inhibited the increase of [Ca2 +] i induced by histamine, serotonin and glutamate. When external calcium was zero +0.1 mmol·L-1 of ethacryl, TMB830 μmol·L-1 could significantly reduce resting [Ca2 +] i, while TMB830 μmol·L-1 could almost completely block histamine and 5  serotonin increases the role of [Ca2 +] i. CONCLUSION: TMB8 can reduce the resting [Ca2 +] i in cultured basilar artery smooth muscle and inhibit the increase of [Ca2 +] i induced by His, 5HT and Glu.