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引入固定化波吉卵囊藻(Oocystis borgei)和微绿球藻(Nannochloris oculata)于凡纳对虾(Litop Penaeus vannamei)养殖环境中,检测水体、对虾胃和后肠中弧菌的数量变化,研究固定化微藻对虾池弧数量动态影响。结果表明:波吉卵囊藻培养液中9d后不能检测出弧菌,微绿球藻培养液中15d后不能检测出弧菌。引入固定化波吉卵囊藻和微绿球藻的褐藻胶藻珠能抑制弧菌的生长,实验组养殖水体、对虾胃和后肠中弧菌的数量都比对照组低;抑制效果是固定化波吉卵囊藻和微绿球藻混合>固定化波吉卵囊藻>固定化微绿球藻;试验后期实验组弧菌的数量明显低于试验前期。试验期间固定化波吉卵囊藻和微绿球藻的生物量分别增加了约10倍和17倍,证明它们的生理活性不会因固定化而受干扰。因此,固定化微藻可应用于虾池微生态调控防病。
The introduction of immobilized Oocystis borgei and Nannochloris oculata into Litop Penaeus vannamei culture environment was used to detect the changes of Vibrio species in water, shrimp stomach and hindgut, Dynamics of shrimp pond arc number with immobilized microalgae. The results showed that Vibrio can not be detected after 9 days in the culture solution of Oocysts, and Vibrio can not be detected after 15 days. Alginate-alginate beads, which were introduced into oocysts and micro-algae, could inhibit the growth of Vibrio. The numbers of vibrios in shrimp’s stomach and hindgut were lower than those in control group. The inhibitory effect was fixed Ovocryx Boehmeria and Micrococcoclaspids> Immobilized Bojimena> Immobilized Micrococcus nodulis; The number of Vibrio in the experimental group was significantly lower than that in the pre-test. During the experiment, the biomass of the oocysts immobilized with Bojhi and Micrococcus increased about 10-fold and 17-fold, respectively, demonstrating that their physiological activities are not disturbed by immobilization. Therefore, immobilized microalgae can be applied to the micro-ecological regulation and prevention of shrimp ponds.