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将优质、抗纹枯病的高秆供体Tarom Molaii和Binam导入半矮秆IR64和特青背景,培育品质性状回交选择构建的4个导入系群体IR64/Tarom Molaii、特青/Tarom Molaii、IR64/Binam和特青/Binam,定位了影响水稻抗纹枯病病级(disease scale,DS)、相对病斑高度(relative lesion height,RH)和株高(plantheight,PH)的QTL。结果表明,4个导入系群体的DS与RH高度正相关,两者与PH呈显著负相关。导入系后代各性状均呈现超亲分离,出现抗性明显优于双亲的抗病个体,其中40%左右属半矮秆抗病类型。采用单向方差分析,在这4个群体中分别定位到10、8、8和6个影响3个性状的QTL,多数基因座上降低DS和RH即增强抗病性同时增加株高的等位基因均来自两个供体。未在同一供体两个不同背景下检测到影响3个性状的相同QTL,表明抗纹枯病QTL表达有明显的遗传背景效应。PH与DS及PH与RH被定位在同一个显著标记位点的QTL数分别占两个性状QTL总数的38%和52%,表明水稻纹枯病抗性与株高关系密切,两者存在许多连锁位点。与以往相同群体品质性状QTL的定位结果相比,发现品质性状QTL与抗纹枯病QTL大多分布在染色体的不同区域,彼此独立遗传。对利用目标性状选择导入系定位非目标性状QTL的效果、影响因素及育种应用进行了探讨,强调了目标性状选择导入系对非目标性状QTL发掘及育种应用的重要性。
Tarom Molaii and Binam were introduced into semi-dwarf IR64 and Teqing backgrounds. The four elite transformants (IR64 / Tarom Molaii, Tarom Molaii, IR64 / Binam and Binam, mapped the QTLs that affected rice disease severity (DS), relative lesion height (RH) and plant height (PH). The results showed that there was a significant positive correlation between DS and RH in four inbred lines, and negative correlation between them and PH. All the traits introduced into the offspring had super-pro-segregation, and the resistant individuals were obviously better than the parents, of which about 40% were semi-dwarf resistant types. Using one-way analysis of variance, QTLs affecting 10, 8, 8 and 6 traits affecting 3 traits were located in these 4 populations. Most loci decreased DS and RH to enhance disease resistance and increase plant height Genes are from two donors. The same QTLs affecting three traits were not detected in two different backgrounds of the same donor, indicating that the QTLs for resistance to Sheath blight have a significant genetic background effect. The QTLs for PH and DS, PH and RH were all located at the same significant marker sites, accounting for 38% and 52% of the total QTLs for both traits, respectively, indicating that resistance to sheath blight in rice is closely related to plant height, Chain sites. Compared with the QTLs for QTLs for the same quality traits in the past, it was found that the QTLs for quality traits and QTLs for resistance to sheath blight were mostly distributed in different regions of the chromosomes and were independently inherited from each other. The effects, influencing factors and breeding applications of QTLs for target locus selection of non-target trait loci were discussed, and the importance of QTLs for non-target trait selection and breeding application was emphasized.