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作者报告卡介苗全细胞壁(WCW)和附油的深红奴卡氏菌细胞壁骨架(CWS)及卡介苗 CWS 对小鼠自发性乳腺腺癌或甲基胆蒽诱发的皮下肉瘤的自身移植物的影响。方法:先使完整的细胞破裂,用离心法除去未破裂的细胞及碎屑,然后真空冻干,即制成 WCW。用蛋白分解酶处理WCW,浸出水溶性物质并用乙醚等除去游离的脂肪。除去脂肪后的残余物即CWS。它主要由奴卡分枝酸(卡介苗的CWS 则为分枝酸)、中性糖类、氨基酸及氨基糖类组成。在完全切除动物的肿瘤以
The authors report the effects of BCG (WCW) and oil-eluting CNV cell wall scaffolds (CWS) and BCG CWS on autologous grafts of mouse spontaneous breast adenocarcinoma or methylcholanthrene-induced subcutaneous sarcoma. Methods: Whole cells were first ruptured, unruptured cells and debris were removed by centrifugation and then lyophilized in vacuo to give WCW. WCW is treated with a proteolytic enzyme, the water-soluble substance is leached and the free fat is removed with ether or the like. The residue after the fat removal is CWS. It is mainly composed of slave card branching acid (CWS of BCG is a branched acid), neutral sugars, amino acids and amino sugars. After completely removing the animal’s tumor