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目的 :检测并比较胃癌及癌前病变 p5 3蛋白表达水平 ,探讨p5 3蛋白在胃癌发病中的意义。 方法 :采用间接免疫荧光标记 ,流式细胞术分析。以DNA指数、增殖指数、荧光指数为分析指标。结果 :胃癌、不典型增生及肠上皮化生的荧光指数 (FI)值与正常胃粘膜相比差异均有显著性 (P <0 0 5 ) ,前三者与浅表性胃炎相比差异也都有显著性 (P <0 0 5 )。不同病变间的比较 ,可见胃癌FI高于不典型增生及肠上皮化生 (P <0 0 5 )。不典型增生 p5 3蛋白阳性率为 2 7% ,胃癌为 6 8% ,在不典型增生及胃癌病例中 ,其异倍体的FI值、增殖指数 (PI)值和 p5 3蛋白阳性率与二倍体者相比差异都有显著性 (P <0 0 5 )。 结论 :胃癌组织 p5 3蛋白的表达水平高于癌前病变及正常胃粘膜组织 ,随病变向恶性转化 ,p5 3蛋白、PI及异倍体率均增高。检测 p5 3蛋白表达水平对胃癌的诊断具有一定意义
Objective: To detect and compare the expression of p53 protein in gastric cancer and precancerous lesions, and to explore the significance of p53 protein in the pathogenesis of gastric cancer. Methods: Indirect immunofluorescence labeling, flow cytometry analysis. Take the DNA index, proliferation index, and fluorescence index as the analysis indicators. Results: The fluorescence index (FI) values of gastric cancer, atypical hyperplasia, and intestinal metaplasia were significantly different from those in normal gastric mucosa (P < 0.05). The difference between the first three and superficial gastritis was also significant. There are significant (P < 0 0 5). Comparing different lesions, we can see that FI in gastric cancer is higher than dysplasia and intestinal metaplasia (P <0 05). The positive rate of p53 protein in atypical hyperplasia was 27% and gastric cancer was 68%. In an atypical hyperplasia and gastric cancer cases, the aneuploid FI, proliferative index (PI) value and p53 protein positive rate were two. The difference in ploidy was significant (P < 0.05). Conclusion : The expression of p53 protein in gastric cancer tissues is higher than that in precancerous lesions and normal gastric mucosa. With the malignant transformation of p53 protein, the rate of p53 protein, PI and heteroploidy increased. Detection of p53 protein expression level has certain significance in the diagnosis of gastric cancer