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Chitosan was modified by conjugating coupling with linolenic acid through the l-ethyl-3-( 3-dimethylami-nopropyyl) carbodiimmide (EDC)-mediated reaction. The degree of substitution 1.8% (i.e. 1.8 linolenic acid group per 100anhydroglucose units) was measured by 1H NMR. The critical aggregation concentration (CAC) of the self-aggregate of hy-drophobically modified chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe.The CAC value in phosphate-buffered saline (PBS) solution (pH7.4) was 5 × 10-2 mg mL-1. The average particle size of self-aggregates of hydrophobically modified chitosan in PBS solution (pH7.4) was 210.8 nm with a unimodal size distributionranging from 100 to 500 nm. Transmission electron microscopy (TEM) study showed that the formation of near sphericalshape nanoparticles has enough structural integrity. The loading ability of hydrophibically modified chitosan (LA-chitosan)was investigated by using bovine serum albumin (BSA) as the model. The loading capacity of self-aggregated nanoparticlesincreases (19.85% ±0.04% to 37.57% ±0.25%) with the concentration of BSA (0.1-0.5mgmL-1).
Chitosan was modified by conjugating coupling with linolenic acid through the l-ethyl-3- (3-dimethylami-nopropyyl) carbodiimmide (EDC) -mediated reaction. The degree of substitution 1.8% (ie 1.8 linolenic acid group per 100 hydhydrollucose units) was measured by 1H NMR. The critical aggregation concentration (CAC) of the self-aggregate of hy-drophobically modified chitosan was determined by the fluorescence intensity of the pyrene as a fluorescent probe. CAC value in phosphate-buffered saline (PBS) solution ( pH 7.4) was 5 x 10-2 mg mL-1. The average particle size of self-aggregates of hydrophobically modified chitosan in PBS solution (pH 7.4) was 210.8 nm with a unimodal size distribution drawing from 100 to 500 nm. Transmission The loading ability of hydrophibically modified chitosan (LA-chitosan) was investigated by using bovine serum albumin (BSA) as the model. The loading capacity of self-aggregated nanoparticles in creases (19.85% ± 0.04% to 37.57% ± 0.25%) with the concentration of BSA (0.1-0.5 mg mL-1).