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作者对慢性渗出性中耳炎(COME)100例和蓝色鼓膜(B ED)13例共113名儿童进行了中耳渗物抗体的研究,患儿年龄为1～11岁。从128耳(COME114耳和BED14耳)经鼓膜切开术抽吸渗液,存-80℃条件下待检。抗原有腺病毒7型Grider株(培育在HEp-2细胞中)、呼吸道合胞体(R S)Long氏株及副流感Ⅱ型病毒(Para2)的Greer株,(均在Vero细胞中培育)。腺病毒抗原提纯按Green和Pi na氏方法进行。R S和Para 2型的核壳蛋白提纯按Sarkkimen氏等方法进行。兔抗人IgG和SIgA的抗血清用乙基氨基纤维素柱层析法提取,并标
The authors studied middle ear osmotole antibody in 100 children with chronic exudative otitis media (COME) and 13 with blue eardrum membrane (B ED), ages 1 to 11 years old. From 128 ears (COME114 ears and BED14 ears) by tympanotomy aspirate exudate, stored at -80 ℃ to be seized. The antigens were the Greer strain of adenovirus Type 7 Grider strain (grown in HEp-2 cells), Respiratory Syncytial (Rs) Long strain and Para2 strain (both cultivated in Vero cells). Adenovirus antigen purification by Green and Pi na’s method. Purification of Rs and Para 2 nucleocapsid proteins was carried out by the method of Sarkkimen et al. Antiserum of rabbit anti-human IgG and SIgA was extracted with ethyl-aminocellulose column chromatography and labeled