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1.菌种生产母种、原种、栽培种选择全营养高氮型菌种,通用培养基按配方一次性制备。母种培养基用18×180毫米的玻璃试管盛装,放入口径22—24厘米高压锅内灭菌40分钟;原种和栽培种培养基用500毫升罐头瓶或500毫升生理盐水瓶盛装,放入太空灭菌包(自制材料费约10元,可盛500—800瓶)内灭菌3—4小时,或置入常压灭菌锅(上蒸锅)内灭菌8—10小时,冷却至常温后取出。接种用新开放型食用菌无菌接种器(自制材料费3—5元),直接将上述培
1. Species production of mother species, original species, cultivated species selection of fully nutritious high nitrogen-type bacteria, common medium preparation formula according to one-time. Mother culture medium with 18 × 180 mm glass test tube, placed in diameter 22-24 cm autoclave sterilization 40 minutes; the original species and culture medium with 500 ml cans or 500 ml saline bottle containing, into Sterilization of the space package (homemade materials costs about 10 yuan, can Sheng 500-800 bottles) within 3-4 hours of sterilization, or placed in an autoclave (on the steamer) sterilization 8-10 hours, cooled to Remove after room temperature. Vaccination with a new open mushroom inocula (homemade materials costs 3-5 yuan), the above-mentioned training