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目的观察腺苷A1受体(adenosine A1 receptor,A1R)是否介导参麦注射液对脑缺血再灌注损伤大鼠的脑保护作用。方法采用大脑中动脉阻塞法(middle cerebral artery occlusion,MCAO)建立脑缺血再灌注损伤模型。将60只模型制备成功大鼠按随机区组原则分为模型组、参麦组、参麦加A1R拮抗剂(1,3-dipropyl-8-cyclopentylxanthine,DPCPX)组、A1R拮抗剂对照组和二甲亚砜溶剂对照组,每组12只,另设假手术组12只。在大鼠脑缺血即刻时,参麦组大鼠腹腔注射参麦注射液15 mL/kg,模型组和假手术组大鼠腹腔注射等量的生理盐水;参麦加A1R拮抗剂组大鼠在注射参麦注射液前30 min腹腔注射DPCPX1 mg/mL;A1R拮抗剂对照组、二甲亚砜溶剂对照组大鼠分别在脑缺血即刻前30 min给予腹腔注射DPCPX(1 mg/kg)和二甲亚砜(1 mL/kg)。再灌注24 h时评估大鼠的神经行为学评分,检测脑梗死体积以及脑梗死半暗带区Bcl-2蛋白表达量。结果与假手术组比较,模型组大鼠神经行为学评分、梗死体积和Bcl-2蛋白表达量均增加(P<0.05);与模型组比较,参麦组大鼠行为学评分和梗死体积明显减少,Bcl-2蛋白表达量增加(均P<0.05);与参麦组比较,参麦加A1R拮抗剂组大鼠行为评分升高,梗死体积明显增加,Bcl-2蛋白表达量明显减少(均P<0.05)。结论 A1R拮抗剂能部分逆转参麦注射液对脑缺血再灌注损伤大鼠的行为学评分,梗死体积和Bcl-2蛋白表达的改善作用,A1R可能介导了参麦注射液对脑缺血再灌注损伤大鼠的脑保护作用。
Objective To investigate whether the adenosine A1 receptor (A1R) mediates the neuroprotective effect of Shenmai injection on cerebral ischemia-reperfusion injury in rats. Methods Cerebral ischemia-reperfusion injury model was established by middle cerebral artery occlusion (MCAO). 60 rats were randomly divided into model group, Shenmai group, Shenmai A1R antagonist group (1,3-dipropyl-8-cyclopentylxanthine DPCPX), A1R antagonist control group and two DMSO control group, each group of 12, another set of sham-operated group of 12. At the moment of cerebral ischemia in rats, Shenmai rats were injected intraperitoneally with 15 mL / kg Shenmai injection, and the rats in the model group and the sham operation group were injected with the same amount of normal saline. Rats in the Shenmai A1R antagonist group DPCPX1 mg / mL was injected intraperitoneally 30 minutes before injection of Shenmai injection. Rats in A1R antagonist control group and dimethyl sulfoxide solvent control group were intraperitoneally injected with DPCPX (1 mg / kg) And dimethyl sulfoxide (1 mL / kg). At 24 h after reperfusion, the neurobehavioral scores of rats were assessed, and the volume of cerebral infarction and the expression of Bcl-2 protein in penumbra of cerebral infarction were detected. Results Compared with the sham operation group, the neurobehavioral score, infarct volume and Bcl-2 protein expression in the model group were significantly increased (P <0.05). Compared with the model group, the scores of the behavior and the infarct volume in the Shen Mai group were significantly (P <0.05). Compared with the Shenmai group, the rats in the Shenmai A1R antagonist group had higher behavioral scores, significantly increased infarct volume, and significantly decreased the expression of Bcl-2 protein (P < All P <0.05). Conclusion A1R antagonist can partially reverse the effects of Shenmai injection on behavior score, infarct volume and Bcl-2 protein expression in rats with cerebral ischemia-reperfusion injury. A1R may mediate the effect of Shenmai injection on cerebral ischemia Brain protective effect of reperfusion injury in rats.