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目的探讨结核感染T细胞免疫检测和蛋白芯片技术,在HIV感染合并结核潜伏感染者中的应用。方法用结核感染T细胞免疫检测(IGRA)和蛋白芯片试剂盒对116例明确诊断HIV感染者,进行γ-干扰素释放含量和IGg抗体检测。结果在HIV感染者中结核感染T细胞免疫检测法(IGRA)检出阳性率为21.6%,蛋白芯片技术检测阳性率为7.8%,且2种方法差异具有统计学意义(χ2=8.6,P<0.01)。按照CD4细胞计数≥200个/μl和CD4细胞计数<200个/μl分组,2组IGRA检出阳性率均高于蛋白芯片法的检出阳性率。IGRA在2组中的检出阳性率差异无统计学意义(P>0.05)。结论 IGRA较适用于HIV/AIDS患者合并结核潜伏感染的筛查。蛋白芯片法不适用于HIV/AIDS患者合并结核潜伏感染的筛查。
Objective To explore the application of T cell immune detection and protein chip technology in tuberculosis infection in HIV infection complicated with tuberculosis latent infection. Methods Totally 116 cases of HIV-positive patients were diagnosed by IGT and protein chip kit, and the content of interferon-γ released and IGg antibody were detected. Results The positive rate of T cell immunoassay (IGRA) was 21.6% among TB patients with HIV infection and 7.8% by protein chip technology (χ2 = 8.6, P < 0.01). According to the CD4 cell count≥200 cells / μl and the CD4 cell count <200 cells / μl, the positive rates of IGRA in both groups were higher than those detected by the protein chip method. There was no significant difference in the positive rate of IGRA between the two groups (P> 0.05). Conclusion IGRA is more suitable for HIV / AIDS patients with latent tuberculosis infection screening. Protein chip method is not suitable for HIV / AIDS patients with tuberculosis latent infection screening.