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本文对芦笋花药培养技术进行了详细研究,研究结果表明:二倍体芦笋花蕾长度为1.5～2.0mm, 四倍体芦笋花蕾长度为2.0～2.5mm时,其诱导效果最好;采用5%高糖浓度可以显著抑制花药体细胞分裂和提高花粉愈伤组织诱导率;PH值为6.0时愈伤组织诱导率最高;接种时间以4月底最好;同一品种不同植株花药愈伤组织诱导率差异显著;适合于二倍体花药愈伤组织诱导的激素配比是NAA1.0mg/L,6-BA1.0mg/ L;适合于四倍体花药愈伤组织诱导的激素配比是2,4-D0.5mg/L,NAA0.5mg/L,6-BA0.1mg/L;愈伤组织器官分化的培养激素适宜配比是6-BA1.0mg/L、NAA0.5mg/L。
In this paper, the anther culture technology of asparagus was studied in detail. The results showed that the flower bud length of diploid asparagus was 1.5-2.0 mm and the flower bud length of tetraploid asparagus was 2.0-2.5 mm. The best induction rate was 5% Sugar concentration significantly inhibited the anther somatic cell division and increased the callus induction rate; the callus induction rate was the highest at pH 6.0; the inoculation time was the best at the end of April; the callus induction rate of different plants of the same variety was significantly different ; Suitable for diploid anther callus induction hormone ratio NAA1.0mg / L, 6-BA1.0mg / L; suitable for tetraploid anther callus induction hormone ratio is 2,4-D0 .5mg / L, NAA0.5mg / L, 6-BA0.1mg / L; callus organ differentiation hormone training ratio is 6-BA1.0mg / L, NAA0.5mg / L.