【摘 要】
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以3T3-L1前脂肪细胞的分化功能为指标,探讨了30种天然药物的减肥活性。方法:3T3-L1前脂肪细胞以诱导分化培养基(含胰岛素5μg/mL、地塞米松1μM、IBMX0.5μM)、含胰岛素(5μg
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以3T3-L1前脂肪细胞的分化功能为指标,探讨了30种天然药物的减肥活性。方法:3T3-L1前脂肪细胞以诱导分化培养基(含胰岛素5μg/mL、地塞米松1μM、IBMX0.5μM)、含胰岛素(5μg/mL)的脂肪细胞培养基以及不含胰岛素的普通脂肪细胞培养基各培养2d,其间添加各种浓度(4、20和100μg/m
Taking the differentiation function of 3T3-L1 preadipocytes as an indicator, 30 kinds of natural medicines were investigated for their slimming activity. Methods: 3T3-L1 preadipocytes were cultured in differentiation medium (containing 5 μg/mL of insulin, 1 μM of dexamethasone, 0.5 μM of IBMX), fat cell culture medium containing insulin (5 μg/mL), and normal adipocytes without insulin. Each medium was incubated for 2 days with varying concentrations (4, 20, and 100 μg/m)
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