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目的研究直肠腺癌及其癌旁组织中PSCA,OCT-4,CD24和CD44v6 4种癌干细胞标记蛋白表达水平及其临床病理意义。方法 50例直肠腺癌和20例癌旁组织手术切除标本常规制作石蜡包埋切片,前列腺干细胞抗原(PSCA),OCT-4,CD24和CD44v6染色方法为EnVisionTM二步免疫组化法。结果直肠腺癌PSCA,OCT-4,CD24和CD44v6表达阳性率明显地高于癌旁组织(均P<0.01);阳性表达的癌旁组织均为呈不典型增生者。癌细胞未侵犯浆膜层,肿块最大径≤5 cm,无淋巴结转移及Duke′s A+B期者的PSCA,OCT-4,CD24和CD44v6表达阳性率明显低于侵犯浆膜层,肿块最大径>5 cm,淋巴结转移及Duke′s C+D期者(P<0.05或P<0.01);4种标记蛋白在直肠腺癌中表达水平呈明显一致性(P<0.01)。结论 PSCA,OCT-4,CD24和CD44v6表达水平可能是反映直肠腺癌的发生、进展、临床生物学行为及患者预后的重要癌干细胞标记蛋白。
Objective To study the expression of four marker proteins of PSCA, OCT-4, CD24 and CD44v6 in rectal adenocarcinoma and its adjacent tissues and their clinicopathological significance. Methods Paraffin-embedded sections were obtained from 50 cases of rectal adenocarcinoma and 20 cases of para-carcinoma tissues. PSCA, OCT-4, CD24 and CD44v6 staining were performed by EnVisionTM two-step immunohistochemistry. Results The positive rates of PSCA, OCT-4, CD24 and CD44v6 in rectal adenocarcinoma were significantly higher than those in para-cancerous tissues (all P <0.01). The positive expressions of PSCA, OCT-4, CD24 and CD44v6 were all atypical hyperplasia. The positive rates of PSCA, OCT-4, CD24 and CD44v6 in non-lymph node metastasis and Duke’s A + B stage were significantly lower than those invaded serosa, the largest mass (P <0.05 or P <0.01). The expression levels of four marker proteins in rectal adenocarcinoma were significantly different (P <0.01). Conclusions The expressions of PSCA, OCT-4, CD24 and CD44v6 may be important cancer stem cell marker proteins that reflect the occurrence, progression, clinical biological behavior and prognosis of rectal adenocarcinoma.