论文部分内容阅读
应用非同位素原位杂交技术及反转录ＰＣＲ（ＲＴ－ＰＣＲ），检测了１１种肿瘤细胞系上ＩＬ－６Ｒα／ＩＬ－６ＲβｍＲＮＡ的表达情况，同时应用Ｗｅｓｔｅｒｎｂｌｏｔ检测了ＩＬ－６Ｒα／ＩＬ－６Ｒβ蛋白的表达。结果表明，１１种细胞系中有６种细胞系检测到ＩＬ－６ＲαｍＲＮＡ的表达，有５种细胞系有ＩＬ－６ＲβｍＲ－ＮＡ的表达，有两种细胞系中均没有表达，在蛋白检测中得到同样的结果。生物学活性检测发现，在细胞系中只有当ＩＬ－６Ｒα／ＩＬ－６Ｒβ亚基同时在细胞中表达，而且表达量具有一定合适比例时，才能对一定浓度的ＩＬ－６刺激产生明显的生物学效应。
Non-isotopic in situ hybridization and reverse transcription-PCR (RT-PCR) were used to detect the expression of IL-6Rα/IL-6Rβ mRNA in 11 tumor cell lines. IL-6Rα/IL-6Rβ protein was detected by Western blot. expression. The results showed that the expression of IL-6Rα mRNA was detected in 6 cell lines of 11 cell lines, and the expression of IL-6RβmR-NA was observed in 5 cell lines. No expression was observed in the two cell lines. The same result. The biological activity test found that only in the cell line when IL-6Rα/IL-6Rβ subunits are simultaneously expressed in the cells, and the expression level has a certain appropriate ratio, it can produce obvious biology for a certain concentration of IL-6 stimulation. effect.