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一、目的:掌握贮藏花粉生命测定的方法,并了解其原理。 二、用具:载玻片、盖玻片、解剖针、毛笔、干燥器、小试管或指形管、脱脂棉或纱布、标签、玻璃铅笔、显微镜、冰箱、大广口瓶等。 三、说明:进行杂交的各种树种常因花期不同或要进行远距离杂交而不得不进行花粉贮藏,因此我们必须掌握花粉贮藏的原理与技术。 为了避免杂交工作失误,在使用远地寄来的花粉或经过一段时间贮藏的花粉之前,有必要先测定一下花粉的生命力,如果花粉生命力已经丧失或只有极微弱的生命力时(有人认为发芽率低于5％者就算极弱)则不采用。此外,在杂交之前,了解一下花粉的生命力也有利于杂交成果的分析与研究。
First, the purpose: to master the method of measuring pollen life, and understand the principle. Second, the appliances: slide, cover glass, dissecting needles, brush, dryer, small test tube or finger tube, absorbent cotton or gauze, labels, glass pencils, microscopes, refrigerators, large mouth bottles. Third, the description: The various species of hybrids are often due to different flowering or to carry out long-distance hybrids and had pollen storage, so we must master the principles and techniques of pollen storage. In order to avoid mistakes in hybridization, it is necessary to determine the pollen’s viability before using the pollen sent from far away or the pollen stored over time. If the pollen vitality has been lost or has only a very weak vitality (some people think that the rate of germination is low 5% even if very weak) is not used. In addition, understanding the vitality of pollen before hybridization is also conducive to the analysis and research of hybridization results.