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地中海贫血是华南地区较常见的一种遗传病,目前尚无有效的治疗方法。为开展该病的产前诊断。杜绝患婴出生,我们参考鞍钢韩安国的盲穿操作,用英国的手术器械,摸索了不用B型超声仪监视的吸取绒毛方法,以期能在我国省市以下基层医院推广。绒毛吸取手术无痛,需时10分钟,在门诊手术室进行。受试者将100名怀孕42～88天需做人工流产的妇女。用注射器抽至20ml负压,每次平均吸到6mg绒毛,成功率81％。受试者无1例术后出血;其中23位术后观察7～10天再行人工流产,其间妊娠均正常,没有自发流产。人流手术后检查胎膜未见异常。DNA提取采用简化方法,一天即可完成。绒毛经匀浆、蛋白酶K消化和氯仿/
Thalassemia is a more common genetic disease in southern China, there is no effective treatment. To carry out the prenatal diagnosis of the disease. To prevent the birth of infants, we refer to the blind wear Han Ansuo Anshun operation, with the British surgical instruments, groping without B-type ultrasound monitoring fluff method, in order to promote the grassroots hospitals in our provinces and cities. Villus suction surgery painless, take 10 minutes in the clinic operating room. Subjects will be 100 pregnant women 42 to 88 days to be abortion. With a syringe pumped to 20ml negative pressure, each with an average of 6mg villi, the success rate of 81%. None of the subjects had postoperative bleeding; 23 of them underwent postoperative artificial abortion for 7-10 days, with normal pregnancy and no spontaneous abortion. Check abortion after abortion no abnormalities. DNA extraction using a simplified method, a day can be completed. Fluff homogenization, digestion with proteinase K and chloroform /