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1978年以来,我们试用黄浆水(作豆腐的废水——豆腐脑滤过液)代替蛋白胨,制作普通细菌培养基。经过反复试验观察,取得了和蛋白胨(北京市生物化学制药厂产生物培养基——牛牌蛋白胨)培养基相同的效果。甚至比后者更好。1979年4月,已代替蛋白胨,正式应用于检验工作。取材和配制黄浆水是本院用卤水点制的豆腐脑的滤过液。取新鲜滤过液于烧瓶内,加棉塞高压灭菌后,放置室内或冰箱内保存备用。配制液体培养基时,取氯化钠0.5克、肉膏0.3克、黄浆水30毫升,加水至100毫升,混匀后,将pH调至7.4～7.6,过滤、分装、灭菌即可。效果观察我们选择甲链、金葡、白葡、大肠和绿脓菌,用黄浆水和蛋白胨两种培养基,在条
Since 1978, we have experimented with yellowing water (tofu wastewater - tofu brain filtrate) instead of peptone to make ordinary bacterial culture medium. After repeated experimental observation, the same effect as that of peptone (Beijing Biochemical Pharmaceutical Factory-Bio-Peptone) medium was obtained. Even better than the latter. April 1979, has replaced the peptone, formally used in inspection work. Drawing and preparation of yellow pulp water is the court system of brine tofu brain filtration solution. Take fresh filtrate in the flask, add tampon autoclave, placed indoors or in the refrigerator to save. Preparation of liquid medium, take 0.5 grams of sodium chloride, 0.3 grams of meat paste, yellow slurry 30 ml, add water to 100 ml, mix, the pH adjusted to 7.4 to 7.6, filtration, packaging, sterilization can be . Effect of observation We choose a chain, gold Pu, white Pu, colon and Pseudomonas aeruginosa, yellow pulp water and peptone two medium,