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OBJECTIVE:To investigate the effect of Bushenyisui Formula on cell apoptosis and positive B cell lymphoma(Bcl-2) in the Brain of rat models of Alzheimer’s disease(AD) induced by beta-amyloid protein(Aβ) and the mechanism underlying the effect.METHODS:Total of 40 SD rats,20 females and 20 males,were randomly assigned to 4 groups,controlled group(A),model group(B),conventional treatment group(C) and Bushenyisui Formula treatment(BYFT) group(D),10 rats in each group.Aβ 1-42 was injected into the bilateral hippocampus of the rats in group B,C and D to create the models of AD.Sham operation was performed on the rats of group A in the same way by injecting equal volume of 0.9% sodium chloride solution into their bilateral hippocampus.5 days after operation,Bushenyisui Formula was intraperitoneally administered at a dose of 450 mg/kg to the rats of group D(QD) for 20 days.Equalvolumeof0.9%sodiumchloridesolution wasintraperitoneallyinjectedintotheratsofgroupB withthesameprocedure.C suspension(20 mg/mL) was intraperitoneally injected into the rats of group B with the same procedure.The number of apoptotic cells in Brain and the positive Bcl-2 were counted.The changes of learning and memory abilities were evaluated usingY-maze.RESULTS:Right after the establishment of the models,group B,C and D compared to group A respectively,the outcomes of Y-maze were significantly different from that of group A,which suggested obvious learning and memory disorder in those groups(P<0.01).After treatment,the times of electronic shocks of group C and D were significantly less than that of group B(P<0.05),and the numbers of apoptotic cells and positive Bcl-2 were significantly different from those of group B,apoptotic sells’ number of group C and D smaller than that of group B and the number of positive Bcl-2 greater than that of group B.CONCLUSION:Bushenyisui Formula could increase the number of Bcl-2 in brain,which improved the function of nervous system pertaining to learning and memory abilities.
OBJECTIVE: To investigate the effect of Bushenisui Formula on cell apoptosis and positive B cell lymphoma (Bcl-2) in the Brain of rat models of Alzheimer’s disease (AD) induced by beta-amyloid protein (Aβ) and the mechanism underlying the effect. METHODS: Total of 40 SD rats, 20 females and 20 males, randomly assigned to 4 groups, controlled group (A), model group (B), conventional treatment group (C) and Bushenisui Formula treatment (BYFT) , 10 rats in each group. Aβ 1-42 was injected into the bilateral hippocampus of the rats in group B, C and D to create the models of AD. Sham operation was performed on the rats of group A in the same way by injecting equal volume of 0.9% sodium chloride solution into their bilateral hippocampus for 5 days after operation, Bushenyisui Formula was intraperitoneally administered at a dose of 450 mg / kg to the rats of group D (QD) for 20 days. Eqvolume of 0.9% sodium chloride solution was inraperitoneally injected on patients with group B withthesameprocedure.C suspensio n (20 mg / mL) was intraperitoneally injected into the rats of group B with the same procedure. The number of apoptotic cells in Brain and the positive Bcl-2 were counted. The changes of learning and memory abilities were evaluated using Y-maze. RESULTS: Right after the establishment of the models, group B, C and D compared to group A respectively, the outcomes of Y-maze were significantly different from that of group A, which suggested obvious learning and memory disorder in those groups (P < 0.01). After treatment, times of electronic shocks of group C and D were significantly less than that of group B (P <0.05), and the numbers of apoptotic cells and positive Bcl-2 were significantly different from those of group B, apoptotic sells’ number of groups C and D smaller than that of group B and the number of positive Bcl-2 greater than that of group B. CONCLUSION: Bushenyisui Formula could increase the number of Bcl-2 in brain, which improved the function of nervous system pertaining to learning and memory a bilities.