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目的:应用纤维支气管镜将结核分枝杆菌(MTB)接种猕猴肺部,建立结核病模型。方法:分别用不同菌量的MTBH37Rv株,Erdman株和临床耐药株、经纤维支气管镜接种实验猴右肺下叶,在规定的时间,观察其结核菌素皮肤试验和胸部X线表现、肺部病理学改变和肺组织细菌载量。结果:所有感染猕猴的结核菌素皮肤试验呈阳性,胸部X线呈现结核样改变,病理解剖显示肺部均有不同程度的病理变化;显微镜下肺组织中可见特征性的结核结节,肺组织中均培养出了数量不等的结核分枝杆菌。结论:经纤维支气管镜将一定量的结核分枝杆菌接种实验猕猴的右肺能成功建立结核病实验猴模型,该方法能为结核疫苗和药物疗效评价平台提供良好的技术支撑。
OBJECTIVE: To establish a tuberculosis model by inoculating Mycobacterium tuberculosis (MTB) into the lungs of lungs with fiberoptic bronchoscopy. Methods: MTBH37Rv strain, Erdman strain and clinical drug-resistant strain were inoculated with different doses of MTBH37Rv strain and clinical drug-resistant strain respectively. The lower right lobe of lung was inoculated by bronchofiberscopy. The tuberculin skin test and chest X-ray findings were observed at the prescribed time. Department of pathology and lung bacterial load. Results: All of the infected monkeys were positive for tuberculin skin test. The chest X-ray showed tuberculosis-like changes. The pathological anatomy showed that the lungs had different pathological changes. The characteristic tubercles nodules and lung tissues The average number of M. tuberculosis cultivated in each. Conclusion: The tuberculosis experimental monkey model can be established successfully by inoculating a certain amount of Mycobacterium tuberculosis into the right lung of experimental monkeys through fiberoptic bronchoscopy. This method can provide good technical support for tuberculosis vaccine and drug efficacy evaluation platform.