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在乙肝、白喉、破伤风、无细胞百日咳（ｒＨＢ－ＤＴａＰ）四联疫苗的研制过程中，我们采用基因工程乙肝疫苗（ＣＨＯ），精制白喉、破伤风类毒素和无细胞百日咳菌苗原液，按不同的稀释液，不同的加入顺序以及不同的含量等制备四联疫苗，并以单价基因工程乙肝疫苗（ｒＨＢ）做对照，进行小鼠试验，接种４周后采血检测抗－ＨＢｓ水平。结果表明，不同配方的四联疫苗中ｒＨＢ与ＤＴａＰ均无干扰，相容性好；抗－ＨＢｓ免疫应答水平与单价苗（ｒＨＢ）无显著性差异（Ｐ＞０．０５），使用生理盐水制备的ｒＨＢ－ＤＴａＰ其抗－ＨＢｓ免疫应答水平较醋酸盐缓冲液者高（Ｐ＜０．０５），ｒＨＢ－ＤＴａＰ中的抗－ＨＢｓ免疫应答水平与ｒＨＢ原液的加入顺序无关（Ｐ＞０．０５）；ｒＨＢ含量以２０μｇ／ｍｌ为宜。
In the development of hepatitis B, diphtheria, tetanus and acellular pertussis (rHB-DTaP) tetanus vaccine, we used the genetic engineering hepatitis B vaccine (CHO), refined diphtheria, tetanus toxoid and acellular pertussis vaccine Different dilutions, different order of addition and different content were prepared, and the monovalent genetically engineered hepatitis B vaccine (rHB) was used as the control. The mice were tested and the blood samples were collected 4 weeks after the vaccination to detect the level of anti-HBs. The results showed that rHB and DTaP did not interfere with tetraploid vaccine, and the compatibility of anti-HBs vaccine was not significantly different from that of monovalent vaccine (rHB) (P> 0.05) Of rHB-DTaP had higher anti-HBs immune response than acetate buffer (P <0.05). The level of anti-HBs immune response in rHB-DTaP was not related to the order of addition of rHB solution (P> 0.05). 05); rHB content of 20μg / ml is appropriate.