论文部分内容阅读
Objective To observe the effect of C-reactive protein (CRP) on platelet endothelial nitric oxide synthase (eNOS) activity. Methods Peripheral venous blood was collected, platelets were isolated with gel-filtration chromatography and incubated with histamine, L-NAME and different concentrations of CRP(5, 10, 25, 50, 100μg) for 30 min, then eNOS activity was measured as formation of 3H-L-citrulline from 3H-L-arginine. Results Platelet
Objective To observe the effect of C-reactive protein (CRP) on platelet endothelial nitric oxide synthase (eNOS) activity. Methods Peripheral venous blood was collected, platelets were isolated with gel-filtration chromatography and incubated with histamine, L-NAME and different concentrations of CRP (5, 10, 25, 50, 100 μg) for 30 min, then eNOS activity was measured as formation of 3H-L-citrulline from 3H-L-arginine.