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表面等离子共振技术是用来研究分子相互作用有力而灵敏的工具.近年来随着技术的发展,表面等离子共振仪出现一些新的功能和应用,广泛应用于药物研发的多个阶段.表面等离子共振仪可以采集回收钓靶的成分,提供丰富而深入的结合信息,包括亲和力、特异性、动力学、浓度和识别共价/变构/竞争的结合行为.这篇综述重点介绍了表面等离子共振技术的原理、样品类型、检测范围、实验方法、优势、局限性、以及药物研发过程中最新的应用进展.“,”Surface plasmon resonance (SPR) technology is a powerful and sensitive tool for investigating molecular interactions.Technical improvements in recent years have brought new functionality to SPR apparatuses that can be widely applied at multiple stages of the drug discovery process.The technology allows users to conduct ligand fishing and offers rich and in-depth information regarding the affinity,specificity,kinetics,concentration and identification of covalent/allosteric/competitive binding behaviors.The present review highlights the principle,sample types,detection ranges,experimental methods,strengths,limitations,and the latest research progress on the application of SPR technology in the drug discovery process.