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目的：以Ｋ５６２细胞为阴性对照，观察ＳＵＮＥ－１和Ｂ９５８两种含ＥＢＶ－ＢＨＲＦ１基因的肿瘤细胞株在２×１０６ｍｏｌ／Ｌ地塞米松（ｄｅｘａｍｅｔｈａｓｏｎｅ，ＤＥＸ）作用１８ｈ后，细胞内ＥＢＶ－ＢＨＲＦ１基因转录水平的改变。方法：细胞ＲＮＡ的提取、ＲＮＡ的点杂交（Ｄｏｔｂｌｏｔ）及反转录ＰＣＲ（ＲＴ－ＰＣＲ）。结果：上述两种肿瘤细胞在２×１０６ｍｏｌ／Ｌ地塞米松作用１８ｈ等诱导细胞凋亡的条件下，细胞内ＥＢＶ－ＢＨＲＦ１基因的转录水平（ｍＲＮＡ）明显提高。结论：本结果初步显示ＥＢＶ－ＢＨＲＦ１基因的转录与宿主细胞抵抗凋亡的关系密切。
Objective: K562 cells as a negative control, observed SUNE-1 and B95 8 two EBV-BHRF1-containing tumor cell lines in 2 × 10 6mol / L dexamethasone (DEX) after 18h, intracellular Change of EBV-BHRF1 gene transcription level. Methods: Cell RNA extraction, RNA dot blot and reverse transcription polymerase chain reaction (RT-PCR). Results: The transcription level (mRNA) of EBV-BHRF1 in the above two kinds of tumor cells was significantly increased under the condition of 2 × 106mol / L dexamethasone for 18 hours and so on. Conclusion: Our results showed that the transcription of EBV-BHRF1 gene is closely related to the resistance of host cells to apoptosis.