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目的 研究单核细胞趋化蛋白 - 1(MCP- 1)在 B3型柯萨奇病毒 (CVB3)诱导病毒性心肌炎 (VMC)发病中的作用。方法 构建小鼠 MCP- 1真核表达质粒 p VM。 CVB3腹腔注射雄性 BAL B/c小鼠 ,随机分四组 :单独 CVB3感染 VMC组 (A组 )、10 μg过表达 MCP- 1组 (B组 )、4 0 μg过表达 MCP- 1组 (C)和空质粒对照组 (D组 )。并设未感染生理盐水对照组 (E组 )和未感染过表达组 (F组 )。比较各组小鼠心脏重量和体重的比值 (HW/BW)、心肌组织病理学积分 (PS)、心肌组织病毒载量、血清 CK- MB水平。结果 与 E组相比 ,A组的 HW/BW、PS、血清 CK- MB和心肌组织病毒载量均有明显改变。表明 CVB3感染后小鼠出现一系列的体征改变。与 A组相比 B组的 HW/BW、PS、血清 CK- MB和心肌组织病毒载量均未呈明显改变。但 C组 CVB3载量明显降低 (P<0 .0 5 ) ,血清 CK- MB水平升高 (P<0 .0 1) ,而 HW虽然升高(P<0 .0 5 ) ,但是 HW/BW未呈明显的改变 (P>0 .0 5 ) ,PS升高 (P<0 .0 5 )。 D组与 A组相比 ,CVB3载量、血清 CK-MB水平、HW/BW和 PS均未呈明显的改变 (P>0 .0 5 )。 F组的 HW/BW和血清 CK- MB水平与 E组相比未呈现显著性差异 ,而 PS升高 ,但低于 A组 (P<0 .0 1)。结论 单独在心肌组织过表达 MCP- 1仅引起心肌组织学改变 ,不能
Objective To investigate the role of monocyte chemotactic protein-1 (MCP-1) in the pathogenesis of viral myocarditis (VMC) induced by Coxsackievirus B3 (CVB3). Methods Mouse MCP-1 eukaryotic expression plasmid p VM was constructed. CVB3 was injected intraperitoneally into BALB / c mice, and were randomly divided into four groups: CVB3 infected VMC alone (group A), 10 μg overexpression of MCP-1 (group B) and 40 μg overexpression of MCP-1 ) And empty plasmid control group (group D). Rats in control group (E group) and non-infected group (F group) were established. The ratio of heart weight to body weight (HW / BW), myocardial histopathological score (PS), myocardial tissue viral load and serum CK-MB level in each group were compared. Results Compared with group E, the HW / BW, PS, serum CK-MB and myocardial tissue viral load in group A were significantly changed. The mice showed a series of signs changes after CVB3 infection. Compared with group A, no significant changes were found in HW / BW, PS, serum CK-MB and myocardial tissue viral load in group B. But the level of CVB3 in group C was significantly lower than that in group C (P <0. 05) and the level of CK-MB in serum was increased (P <0. 01) BW was not significantly changed (P> 0.05), PS increased (P <0.05). Compared with group A, group C had no significant change in CVB3 load, serum CK-MB level, HW / BW and PS (P> 0.05). The levels of HW / BW and serum CK-MB in group F were not significantly different from those in group E, but were higher in PS group than those in group A (P <0.01). Conclusions Overexpression of MCP-1 in myocardium alone can only cause changes in myocardial histology, but not in myocardium