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目的:观察活血通督汤对脊髓缺血再灌注损伤关键因子核因子-κB(NF-κB)、血管细胞黏附分子-1(VCAM-1)表达的影响,探讨活血通督汤对脊髓缺血再灌注损伤作用机制。方法:将新西兰家兔随机分为假手术组(8只)、模型组(32只)和活血通督汤组(32只)。3组术前7d均予连续灌胃,活血通督汤组灌服活血通督汤,每日2次,假手术组及模型组予灌服0.9%氯化钠溶液,每日2次。假手术组分离出L3-6动脉但不予夹闭,其余2组分离并夹闭L3-6动脉,制成脊髓缺血再灌注损伤模型。3组分别于术后0.5、1、4、8h取出家兔脊髓,采用免疫组化法和ELISA法检测脊髓组织中的NF-κB、VCAM-1的含量。结果:NF-κB在同一时点表达,假手术组最少,模型组最高;与假手术组相比,其余2组各时点NF-κB都明显升高(P<0.01);但活血通督汤组的表达水平显著低于模型组(P<0.05,P<0.01);VCAM-1在同一时点,假手术组表达最少,模型组表达最高;与假手术组相比,其余2组各时点VCAM-1的表达显著升高(P<0.01);血管表达率情况观察:假手术组始终无阳性血管表达,0.5h时3组皆无阳性血管表达,1、4、8h模型组阳性血管表达率明显高于活血通督汤组(P<0.05,P<0.01)。结论:活血通督汤能有效改抑制NF-κB、VCAM-1的表达,从而减轻炎性反应,以期达到减轻脊髓缺血再灌注损伤。
Objective: To observe the effect of Huoxuetongtang on the expression of nuclear factor-κB (NF-κB) and vascular cell adhesion molecule-1 (VCAM-1), a key factor of spinal cord ischemia-reperfusion injury, Reperfusion injury mechanism. Methods: New Zealand rabbits were randomly divided into sham operation group (n = 8), model group (n = 32) and Huoxuetongsuotang group (n = 32). The rats in the three groups were given continuous gavage 7 days before operation. Huoxue Tongsu Tang group was fed with Huoxuetongsu Decoction 2 times a day. Sham operation group and model group were given 0.9% sodium chloride solution twice daily. The sham operation group was separated from the L3-6 artery but not occluded. The other two groups separated and occluded the L3-6 artery to establish a spinal cord ischemia-reperfusion injury model. The spinal cord of rabbits was removed at 0.5, 1, 4 and 8 hours after operation respectively. The contents of NF-κB and VCAM-1 in spinal cord tissue were detected by immunohistochemistry and ELISA. Results: Compared with the sham-operated group, the expression of NF-κB was significantly increased at the same time point in sham operation group and model group (P <0.01) (P <0.05, P <0.01). VCAM-1 at the same time point had the lowest expression in sham operation group and the highest expression in model group. Compared with sham operation group, the other two groups The expression of VCAM-1 was significantly increased at the time point (P <0.01). The observation of vascular expression rate showed that there was no positive expression of vascular in sham operation group, no positive expression in 0.5h group, positive expression in 1, The rate of vascular expression was significantly higher than that of Huoxuetongdu Tang (P <0.05, P <0.01). Conclusion: Huoxuetongdu decoction can effectively inhibit the expression of NF-κB and VCAM-1, thereby reducing the inflammatory reaction, in order to reduce the spinal cord ischemia-reperfusion injury.