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目的 研究丙型肝炎病毒非结构蛋白 5A(HCVNS5A)对肿瘤坏死因子α(TNFα)诱导HepG2细胞凋亡的抑制作用。方法 设计HCV 1bNS5A区基因片段的特异引物 ,以逆转录巢式PCR方法扩增NS5A基因片段 ,并将其进行TA克隆 ,对阳性克隆进行酶切与序列鉴定 ;构建NS5A基因表达载体 ;通过Lipofe
Objective To study the inhibitory effect of hepatitis C virus non-structural protein 5A (HCVNS5A) on HepG2 cell apoptosis induced by tumor necrosis factor α (TNFα). Methods Specific primers of HCV 1b NS5A gene were designed and amplified by reverse transcription nested polymerase chain reaction (PCR). The NS5A gene fragment was amplified and cloned into TA. The positive clones were digested and sequenced. The NS5A gene expression vector was constructed. Lipofe