论文部分内容阅读
我所于1978年3月进行杉木组织培养法试验,现已获得由杉木茎尖所形成的完整植株23株,高1.6—8厘米,根长0.5—5厘米,同时也获得了一批杉木组织器官的分化苗。二、材料和方法材料:取杉木茎尖,经0.1%升汞表面消毒10分钟,再用无菌水冲洗数次。在无菌条件下,用小镊子剥去顶芽外包着的叶片,即可看到透明的半圆球状的生长点。用无菌刀片切取2—3毫米长的生长点,接种在人工培
I was in March 1978 fir tissue culture test, has been obtained from the fir shoot tip formed by the integrity of the plant 23, 1.6-8 cm high, 0.5-5 cm in root length, but also obtained a group of fir tissue Differentiation of organs. Second, materials and methods Materials: Take the fir shoot tip, sterilized by 0.1% mercuric chloride for 10 minutes, and then rinsed several times with sterile water. Under aseptic conditions, the tips of the top buds are peeled off with a pair of small tweezers to reveal the clear, semi-spherical growth points. Use a sterile blade cut 2-3 mm long growing point, inoculated in artificial training