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目的:探讨尿激酶型纤溶酶原激活物(uPA)与其受体uPAR及丝裂原活化蛋白激酶p38在子宫内膜癌组织的表达及相关性,并结合临床病理特征分析其在子宫内膜癌浸润、侵袭转移过程中的作用。方法:采用免疫组织化学方法(PV-6000二步法)检测12例正常子宫内膜(对照组)、20例子宫内膜增生组织(内膜增生组)及50例子宫内膜癌组织(内膜癌组)中uPA、uPAR及p38的表达,并研究其相关性,分析探讨各因子与子宫内膜癌临床病理分期、组织学分级、组织学类型等的关系。结果:(1)内膜癌组患者uPA、uPAR、p38阳性表达率均显著高于内膜增生组及对照组,差异有统计学意义(P<0.05);内膜增生组与对照组差异无统计学意义(P>0.05);(2)内膜癌组患者uPA和uPAR、p38的表达与临床病理分期、组织学分级、肌层浸润深度及有无淋巴结转移有关,差异有统计学意义(P<0.05);而与患者病理类型不相关(P>0.05);(3)子宫内膜癌组织uPA与uPAR的表达呈正相关(r=0.389,P<0.05);uPA与p38的表达也呈明显正相关(r=0.353,P<0.05)。结论:uPA与uPAR在子宫内膜癌发生发展侵袭转移过程中起着协同作用,p38MAPK信号通路在此过程中可能参与调节uPA,促进癌细胞浸润转移,因此uPA、uPAR、p38可能成为推测子宫内膜癌预后的重要参考指标。
Objective: To investigate the expression and correlation of urokinase-type plasminogen activator (uPA) and its receptor uPAR and mitogen-activated protein kinase p38 in endometrial carcinoma and its clinical significance in endometrial Cancer invasion, invasion and metastasis of the role. Methods: 12 cases of normal endometrium (control group), 20 cases of endometrial hyperplasia (endometrial hyperplasia) and 50 cases of endometrial carcinoma (control group) were detected by immunohistochemistry (PV-6000 two steps method) The expression of uPA, uPAR and p38 in the membrane cancer group and its correlation were analyzed. The relationship between the factors and clinical pathological stage, histological grade and histological type of endometrial carcinoma was analyzed. Results: (1) The positive rates of uPA, uPAR and p38 in endometrial carcinoma group were significantly higher than those in intimal hyperplasia group and control group (P <0.05). There was no significant difference between intimal hyperplasia group and control group (2) The expression of uPA, uPAR and p38 in endometrial carcinoma patients was related to the clinicopathologic stage, histological grade, depth of myometrial invasion and lymph node metastasis (P> 0.05) (P <0.05), but not with pathological types (P> 0.05). (3) The expression of uPA and uPAR in endometrial carcinoma was positively correlated (r = 0.389, P <0.05) There was a significant positive correlation (r = 0.353, P <0.05). Conclusion: uPA and uPAR play a synergistic role in the invasion and metastasis of endometrial carcinoma. The p38MAPK signaling pathway may be involved in the regulation of uPA and promote the invasion and metastasis of cancer cells. Therefore, uPA, uPAR and p38 may be the predictors of intrauterine Membrane cancer prognosis of an important reference index.