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薄层色谱用于分离痕量金属比纸色谱所需的展开时间更短,尤其是圆形薄层色谱,具有快速、简便、灵敏度高对设备要求低的优点。本实验通过对用圆形薄层色谱法分离铜、镍的实验条件的试验(薄层板活化时间、活化湿度展,开剂组成等),从而确定实验步骤,以获得最佳分离效果。圆形薄层色谱分离铜和镍,是用硅胶作支撑体,涂布于玻璃板上,固定相是硅胶上吸收的水份,流动相是含盐酸的丙酮-乙酰丙酮溶液(丙酮:乙酰丙酮:4M盐酸=91:4:5)。根据它们在两相中分配比的差
Thin-layer chromatography is used to separate trace metals faster than paper chromatography, especially circular thin-layer chromatography, with the advantages of being fast, easy, and sensitive, and requiring low equipment. In this experiment, we determined the experimental procedure to get the best separation effect by using the experimental conditions of separation of copper and nickel by TLC (the activation time of the lamellae, the development of the activated humidity, the composition of the deinking agent). Circular thin-layer chromatography Separation of copper and nickel, using silica gel as a support, coated on a glass plate, the stationary phase is the absorption of water on silica gel, the mobile phase is hydrochloric acid acetone - acetylacetone solution (acetone: acetylacetone : 4M hydrochloric acid = 91: 4: 5). According to their difference in the distribution of the two phases