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以3年生平邑甜茶[Malus hupehensis(Pamp)Rehd.var.pinyiensis Jiang]盆栽树为材料,通过 2,4-D水溶液灌根处理,探讨了新根(延长根和吸收根)细胞死亡及其抗凋亡基因的表达特征。结果表明,在60mg·L-12,4-D处理后的70d内,新根细胞死亡量和类 caspase3/7(半胱天冬酶)活性先上升后下降,ATP含量和根系活力的变化与之相反。平邑甜茶抗凋亡基因 MhBAG、MhBI-1 与 MhHSP70 在新根中均受 2,4-D 诱导,随着2,4-D处理时间的延长,MhBAG基因的表达水平先下降后逐渐升高,MhBI-1与MhHSP70则先升高后下降。在2,4-D处理下,吸收根细胞死亡量与根系活力的变化幅度明显高于延长根,吸收根MhBI-1与MhHSP70表达量的早期升高幅度大于延长根,表明吸收根细胞对2,4-D 的敏感性高于延长根;但在基因表达高峰时,延长根抗凋亡基因表达量的升高倍数明显高于吸收根,暗示延长根比吸收根有更强的抗凋亡能力。
The pots of three-year-old Malus hupehensis (Pamp) Rehd.var.pinyiensis Jiang were used as the materials to study the cell death of new roots (prolonged roots and absorbed roots) and their Anti-apoptotic gene expression characteristics. The results showed that the new root cell death and caspase 3/7 (caspase) activity increased first and then decreased, and the content of ATP and root activity in the first 70 days after treatment with 60 mg · L-12,4-D The opposite. The anti-apoptotic genes MhBAG, MhBI-1 and MhHSP70 were all induced by 2,4-D in new roots. With the prolongation of 2,4-D treatment, the expression level of MhBAG decreased firstly and then gradually increased , MhBI-1 and MhHSP70 first increased and then decreased. Under 2,4-D treatment, the changes of root cell death and root vigor were significantly higher than that of the elongation roots. The early increase of MhBI-1 and MhHSP70 expression in roots was greater than that in roots, , But the sensitivity of 4-D was higher than that of elongation root. However, at the peak of gene expression, the increasing fold of prolonged root anti-apoptotic gene expression was significantly higher than that of absorbing root, suggesting that the elongation root has stronger anti-apoptosis than the absorbing root. ability.