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在水稻幼苗被放平后,地上部重力响应部位(地上部基部)的上半部生长比下半部生长慢,从而导致地上部基部发生向上弯曲,发生不对称生长的向重性反应。通过抑制差减杂交(SSH)实验发现,水稻地上部基部向重性弯曲生长过程中,反复糖基化多肽OsRGP1和蔗糖合成酶OsSuS在基部上下部有不对称表达。我们还采用Realtime PCR开展进一步研究。实验结果显示OsRGP1和OsSuS的表达受到IAA的调控。通过生物信息学分析也发现OsRGP1和OsSuS的启动子上存在着生长素的调控元件,生长素极性运输抑制剂TIBA处理能够消除OsRGP1和OsSuS在向重性过程中的不对称表达,这进一步说明向重性过程中不对称分布的生长素介导了OsRGP1和OsSuS基因的不对称表达。另外,向重性反应过程中,检测到基部下半部的己糖浓度增加。因此,OsRGP1的不对称表达可能有助于下半部细胞壁多糖的积累,进而促进了下半部细胞壁的扩张。OsSuS不对称表达也有可能会引起己糖在上下半部的不对称分布。己糖和细胞壁多糖在下半部的积累可能在下部细胞扩张从而导致向重性弯曲生长中起一定的作用。
After the rice seedlings were laid flat, the growth of the upper half of the above-ground gravity response site (above ground above the base) was slower than that of the lower half, resulting in upward bending of the ground above ground and asymmetric growth to severe reaction. By SSH, we found that OsRGP1 and OsSuS were asymmetric in the upper and lower basal part of the rice plant during the growth of the ground above the ground. We also use Realtime PCR for further research. The experimental results show that the expression of OsRGP1 and OsSuS is regulated by IAA. Bioinformatics analysis also showed that there are auxin regulatory elements on the promoters of OsRGP1 and OsSuS. The auxin polar transport inhibitor TIBA treatment can eliminate the asymmetric expression of OsRGP1 and OsSuS in the process of grazing, which further explains The asymmetric distribution of auxin to the major processes mediates the asymmetric expression of OsRGP1 and OsSuS genes. In addition, the hexose concentration in the lower half of the base was detected increased during the heavy reaction. As a result, the asymmetric expression of OsRGP1 may contribute to the accumulation of cell wall polysaccharides in the lower half, thereby contributing to the expansion of the lower cell wall. Asymmetric expression of OsSuS may also cause the hexose to be asymmetrically distributed in the upper and lower halves. Accumulation of hexose and cell wall polysaccharides in the lower half may dilate in the lower cells leading to a role in the development of severe bending.