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目的探讨中药“热毒清”抗人巨细胞病毒（ＨＣＭＶ）的实验和临床效果及作用机制。方法采用定量ＲＴＰＣＲ方法，检测中药“热毒清”干预后感染细胞ＨＣＭＶ晚期ｍＲＮＡ的表达水平，观察感染细胞的病变进展，并在体外实验的基础上，将“热毒清”应用于ＨＣＭＶ活动性感染孕妇的临床治疗，测定治疗前后血清肿瘤坏死因子α（ＴＮＦα）和白细胞介素２（ＩＬ２）的水平。结果“热毒清”对ＨＣＭＶ晚期ｍＲＮＡ以及ＨＣＭＶ所致细胞病变有明显的抑制作用。临床观察发现，ＨＣＭＶＩｇＭ阳性孕妇和育龄妇女ＴＮＦα较对照组显著升高，而ＩＬ２则显著下降；“热毒清”治疗后ＴＮＦα和ＩＬ２迅速恢复至正常水平，ＨＣＭＶＩｇＭ转阴率为７２９％，显著高于未治疗组１３６％的自然转阴率。结论血清ＴＮＦα水平与体内ＨＣＭＶ的活动状态有关，ＨＣＭＶ对机体有免疫损伤效应，“热毒清”能够提高机体的免疫功能，在体内外对ＨＣＭＶ均有显著的抑制作用，是治疗ＨＣＭＶ活动性感染，尤其是孕期感染安全有效的药物。
Objective To explore the experimental and clinical effects and mechanism of the Chinese herbal medicine “Huan Du Qing” against Human Cytomegalovirus (HCMV). METHODS: Quantitative RTPCR was used to detect the expression level of advanced HCMV mRNA in infected cells after intervention with Chinese herbal medicine “Huan Du Qing”. The lesion progress of infected cells was observed. Based on the in vitro experiments, “Hot Duster” was applied to HCMV. Clinical treatment of pregnant women with active infection, serum levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) before and after treatment were measured. The results showed that “Hot Toxic Detoxification” had a significant inhibitory effect on HCMV late mRNA and HCMV-induced cytopathic effect. Clinical observations found that TNF-α in HCMV-IgM-positive pregnant women and women of childbearing age was significantly higher than in controls, while IL 2 was significantly decreased; TNF-α and IL 2 rapidly recovered to normal levels after treatment with “hot-toxic clearing”. The negative rate of HCMV-IgM was 72.9%, which was significantly higher than the natural negative rate of 13.6% in the untreated group. Conclusion The level of serum TNFα is related to the active state of HCMV in vivo. HCMV has an immune-damage effect on the body. “Hot Toxin” can improve the immune function of the body and has a significant inhibitory effect on HCMV both in vitro and in vivo. It is an effective treatment for HCMV. Sexual infections, especially those that are safe and effective during pregnancy.