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目的 观察大鼠星形胶质细胞和视网膜节细胞轴突对视神经挤压伤的反应 .方法 用免疫组织化学染色法和计算机辅助免疫组织化学技术研究视神经连续纵向切片 ,定量分析挤压伤后大鼠视神经细胞反应 .结果 挤压伤后 ,视神经轴突标志物 NF- 16 0 ,星形胶质细胞标志物神经胶质纤维酸性蛋白 (GFAP)在直接机械损伤区活力降低并在其后数周内保持低表达 . 3wk时损伤近端神经段 GFAP的光密度为正常的 1.7倍 .在实验观察的 3wk期间挤压伤区近端的 NF-16 0染色明显高于远端 .结论 星形胶质细胞从位于胶质瘢痕和远端之间的损伤区消失可能是视网膜节细胞轴突不能伸长通过损伤区的原因 .
Objective To observe the response of rat astrocytes and retinal ganglion neurons to optic nerve crush injury.Methods The longitudinal sections of the optic nerve were studied by immunohistochemical staining and computer-aided immunohistochemistry to quantitatively analyze the effects of crush injury Rat optic nerve cell response.Results After crush injury, the optic nerve axon marker NF-16 0 and astroglial marker glial fibrillary acidic protein (GFAP) decreased in direct mechanical injury area and decreased in the following weeks While the expression of GFAP in proximal nerve segment was 1.7 times normal at 3wk.NF-16 0 staining at the proximal end of crush injury zone was significantly higher than that at the distal end during the 3wk observation period.Conclusion Astrocytes Disappearance of stromal cells from the lesion located between the glial scar and the distal end may be responsible for the inability of the axon of the retinal ganglion to elongate through the lesion.