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应用聚合酶链反应（ＰＣＲ）技术，配合限制片段长短多态现象（ＲＦＬＰ）分析，对２２例食管癌病人３号染色体短臂３ｐ１４～２４，１７号染色体短臂１７ｐ１３．１杂合缺失（ＬＯＨ）进行了测定。结果显示：３ｐ２４、３ｐ２１、３ｐ１４和１７ｐ１３．１位点ＬＯＨ检出情况分别为８／１１、２／４、１／９和６／６，其中３ｐ２４和１７ｐ１３．１位点的ＬＯＨ较高，提示这一位点ＬＯＨ的测定有可能成为食管癌早期诊断的分子标志。实验发现，在３ｐ２４ＥＡｐＭＤ位点的ＬＯＨ检测中，哈萨克族杂合缺失为５／５，汉族为２／４，维吾尔族为１／２，这是否与种族遗传有关，有必要扩大病例进一步研究。
Polymerase chain reaction (PCR) technique was used in conjunction with restriction fragment length polymorphism (RFLP) analysis to detect the short-arm 17p13.1 heterozygous deletion of chromosome 3 short arm 3p14-24 and chromosome 17 in 22 esophageal cancer patients. Measurements were made. The results showed that the loci detected by 3p24, 3p21, 3p14 and 17p13.1 loci were 8/11, 2/4, 1/9 and 6/6, respectively, and the loci of 3p24 and 17p13.1 loci were higher. This one-point measurement of LOH may become a molecular marker for the early diagnosis of esophageal cancer. It was found that in the LOH test of the 3p24EApMD locus, the Kazakh heterozygous deletion was 5/5, the Han was 2/4, and the Uygur was 1/2. Whether this is related to ethnicity, it is necessary to expand the case for further study.