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以放射自显影方法研究了AT-1727对小鼠S_(180)(腹水型)细胞动力学的影响。腹腔注射AT-1727 50 mg/kg后标记指数由未给药时的31.1％逐时下降,24小时后降到14.5％,对秋水仙碱阻断后的标记有丝分裂指数也有明显抑制,说明药物有减少S期细胞和延缓S和/或G_2期细胞进入M期的作用。AT-1727可使分裂细胞受损伤,表现为染色体凝聚、粘连,分裂被阻于中期。药物有抑制细胞对DNA前体物的掺入的作用,对G_0期细胞却无明显损伤作用。说明该药为一细胞周期特异性药物。
The effect of AT-1727 on the cell kinetics of S_ (180) (ascites) in mice was studied by autoradiography. The labeling index of AT-1727 50 mg / kg was decreased from 31.1% in the non-administration group to 14.5% in 24 hours after the intraperitoneal injection of AT-1727. The marked mitotic index after the blocking of colchicine was also significantly inhibited Reduce S phase cells and delay the entry of S and / or G2 phase cells into the M phase. AT-1727 can divide the cell damage, manifested as chromosome aggregation, adhesion, division was blocked in the metaphase. Drugs have the effect of inhibiting the incorporation of DNA into DNA precursors, but have no obvious effect on G 0 phase cells. Indicating that the drug is a cell cycle-specific drugs.