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利用ISSR分子标记技术,对采集自安徽省滁州市天然次生林——琅琊山国家森林公园和宣城市人工马尾松纯林——麻姑山林场球孢白僵菌种群的遗传多样性水平和种群遗传结构进行了比较分析。用7个引物分别对2个种群共222株球孢白僵菌菌株进行扩展,共得到58个清晰的扩增位点,其中多态位点56个,多态位点百分率(PPB)为96.55%,Nei’s基因多样性(H)为0.2993,Shannon信息指数(I)为0.4593,种群间的基因分化系数(Gst)为0.1283,基因流Nm=3.3984;可以看出,2个种群间的基因流较小,遗传分化较大,为12.83%,这可能是由于人为选择和基因流障碍引起的;琅琊山白僵菌群体(PPB=96.55%,H=0.2781,I=0.4299)遗传多样性水平较高;麻姑山白僵菌群体(PPB=93.10%,H=0.2552,I=0.3825)遗传多样性水平较低。比较研究了采集自大别山原始林中球孢白僵菌菌株的遗传多样性(PPB=81.00%,H=0.3187,I=0.4782),可见生态环境复杂的原始林中球孢白僵菌遗传多样性最高,天然次生林种群次之,人工纯林种群最小。利用Nei’s遗传距离构建琅琊山和麻姑山白僵菌个体间的遗传关系树状图,由UPGMA聚类分析可知,不同采集地的菌株聚为一类。
ISSR markers were used to analyze the genetic diversity and populations of Beauveria bassiana populations collected from natural secondary forests-Langya Mountain National Forest Park and Xuancheng Artificial Pinus massoniana-Ma Gunshan Forest Farm in Chuzhou City, Anhui Province Genetic structure were analyzed comparatively. A total of 222 strains of Beauveria bassiana were amplified from 2 populations using 7 primers. A total of 58 clear amplification loci were obtained, of which 56 were polymorphic loci with a polymorphic locus percentage (PPB) of 96.55 %, Nei’s gene diversity (H) was 0.2993, Shannon’s information index (I) was 0.4593, the coefficient of genetic differentiation (Gst) was 0.1283 and the gene flow Nm was 3.3984. It can be seen that the gene flow (12.83%), which may be caused by anthropogenic selection and gene flow disorders. The genetic diversity of Beauveria bassiana population (PPB = 96.55%, H = 0.2781, I = 0.4299) The genetic diversity of Beauveria bassiana was significantly lower in Beauveria bassiana (PPB = 93.10%, H = 0.2552, I = 0.3825). The genetic diversity of Beauveria bassiana isolates from the virgin forest in the Dabie Mountains was comparatively studied (PPB = 81.00%, H = 0.3187, I = 0.4782) Population second, the smallest artificial artificial forest population. Using Nei’s genetic distance to construct the tree of genetic relationship between Langya Mountain and Beauveria bassiana individuals, UPGMA cluster analysis showed that the strains collected from different sites were clustered into one category.