论文部分内容阅读
目的探讨油烟中的细颗粒物(PM2.5)对胎鼠肺泡Ⅱ型上皮细胞(AECⅡ)的细胞毒性。方法将1只妊娠18d的SPF级ICR小鼠体内的胎鼠肺组织制成AECⅡ细胞悬液。调整细胞浓度为1×106个/ml,分别加入终浓度为0(溶剂对照,DMEM)、25、50、100、200μg/ml的PM2.5(来源于烹调油烟)溶液,培养12、24和48h后进行MTT试验。调整细胞浓度为5×105个/ml,分别加入终浓度为分别为0(溶剂对照,DMSO)、12.5、25和50μg/ml的PM2.5溶液,培养12、24和48h后测定上清液中超氧化物歧化酶(SOD)活力、丙二醛(MDA)和还原型谷胱甘肽(GSH)含量。结果随着PM2.5染毒浓度的升高和染毒时间的延长,AECⅡ细胞存活率和SOD活力呈下降趋势,MDA含量呈上升趋势;而GSH含量仅随着PM2.5染毒浓度的升高而呈下降趋势。结论油烟中的PM2.5可降低AECⅡ的细胞活力,产生脂质过氧化作用。
Objective To investigate the cytotoxicity of fine particulate matter (PM2.5) in fume to fetal rat alveolar type Ⅱ epithelial cells (AECⅡ). Methods Fetal lung tissue of 1 SPF grade ICR mice on day 18 of pregnancy was made into AEC Ⅱ cell suspension. Adjust the cell concentration to 1 × 106 cells / ml, add PM2.5 (derived from cooking fume) solution with final concentration of 0 (solvent control, DMEM), 25, 50, 100, 200μg / After 48h MTT test. The cell concentration was adjusted to 5 × 10 5 cells / ml. The cells were treated with PM 2.5 at concentrations of 0, 25, 25 and 50 μg / ml, respectively. After 12, 24 and 48 h incubation, the supernatants (SOD) activity, malondialdehyde (MDA) and reduced glutathione (GSH) contents were measured. Results With the increase of PM2.5 concentration and prolongation of exposure time, the survival rate of AECⅡ cells and the activity of SOD decreased while the content of MDA increased. However, the concentration of GSH increased only with the increase of PM2.5 concentration High but declining trend. Conclusion PM2.5 in soot can reduce the cell viability of AECⅡ and produce lipid peroxidation.