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目的：探讨人肝癌染色体ＤＮＡ 的丢失和增加的状态及其在肝癌临床病理及预后中的意义，并为寻找肝癌相关基因提供线索。方法：收集手术切除的肝癌标本３１ 例，采用比较基因组杂交方法对肝癌染色体ＤＮＡ 拷贝数进行检测，同时进行病理观察和随访。结果：在染色体ＤＮＡ 拷贝数的增加中，以１ｑ 的频率最高（８３－９％ ） ，其次为８ｑ（６１－３％ ） ；在染色体ＤＮＡ 的拷贝数的减少中，以１６ｑ 的频率最高（７７－４ ％ ） ，其次为１７ｐ（６１－３ ％ ） 和４ｑ（５１－６ ％ ） 。经统计分析发现，在１ｑ 拷贝数增加的病例中，其半年生存的相对机率比无１ｑ 扩增的病例高１０－５ 倍（ 回归系数β＝ ２－３５，Ｐ＜ ０－０５） ； 有１７ｐ 丢失患者的半年内复发的相对危险性比无１７ｐ 丢失的患者高６－８６ 倍（ 回归系数β＝ １－９３ ， Ｐ＜０－０５） 。此外，１ｑ 的增加与肿瘤大小、有无包膜、坏死程度和ＡＦＰ 浓度等均呈负相关；４ｑ 和１７ｐ 的丢失与肿瘤分化程度均呈负相关；４ｑ 的丢失与８ｑ 的增加呈负相关。这是第一次报道肝癌染色体ＤＮＡ 拷贝数的变化与肝癌临床、病理以及预后之间的关系。结论：在肝癌的发生发展中多个染色体的ＤＮＡ 拷贝数存在着高频率的变化，其中１?
Objective: To investigate the loss and increase of chromosomal DNA in human hepatocellular carcinoma and its significance in the clinicopathological and prognosis of hepatocellular carcinoma, and to provide clues for searching for genes related to hepatocellular carcinoma. METHODS: Thirty-one cases of surgically resected hepatocellular carcinoma were collected. The chromosome DNA copy number of hepatocellular carcinoma was detected by comparative genomic hybridization. Pathological observation and follow-up were performed. Results: In the increase of chromosomal DNA copy number, the frequency with 1q was the highest (83-9%), followed by 8q (61-3%); in the reduction of chromosome DNA copy number, the frequency with 16q was the highest (77 -4 % ), followed by 17p (61-3 %) and 4q (51 - 6 %). After statistical analysis, the relative probability of half-year survival in patients with an increase in 1q copy number was 10-5 times higher than that in patients without 1q amplification (regression coefficient β = 2-35, P < 0-05); there were 17p The relative risk of recurrence in lost patients within six months was 6-86 times higher than those without 17p loss (regression coefficient β = 1-93, P<0-05). In addition, the increase in 1q was negatively correlated with tumor size, presence or absence of envelope, necrosis, and AFP concentration; loss of 4q and 17p was negatively correlated with tumor differentiation; loss of 4q was negatively correlated with increase of 8q. This is the first report of the relationship between the changes in the number of copies of liver cancer chromosomal DNA and the clinical, pathological, and prognosis of liver cancer. Conclusion: There is a high frequency of changes in DNA copy number of multiple chromosomes in the occurrence and development of liver cancer, of which 1?