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研究了不同水热温度下合成的层状双金属氢氧化物(layered double hydroxides,LDHs)对离子交换法制备的MTX/LDHs纳米复合物的影响.并利用透射电镜(TEM)、X-射线衍射(XRD)、MTT等手段,对纳米复合物的结构及载药量、控释-缓释性、生物细胞活性母体等性质进行了系统研究.结果表明,不同LDHs母体对离子交换后复合物的形貌和性质起着至关重要的作用.在磷酸缓冲液中考察了MTX/LDHs纳米复合物的药物控释性能并进行了动力学拟合,结果表明,采用离子交换法制备MTX/LDHs纳米复合物的释药过程是Fick扩散控制的离子交换和粒内扩散过程.最后,采用MTT法探究了MTX/LDHs纳米复合物对肺癌细胞A549增殖的抑制作用,结果表明,复合物较纯的MTX具有更好的抑制癌细胞增殖的作用.
The effects of layered double hydroxides (LDHs) synthesized at different hydrothermal temperatures on the MTX / LDHs nanocomposites prepared by ion-exchange method were investigated and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), MTT and other means, the structure and drug loading of nano-composite, controlled release - sustained release, the biological activity of the mother cell and other properties of the system were studied. The results showed that different LDHs precursor ion exchange complex Morphology and properties of MTX / LDHs nanocomposites were investigated in phosphate buffered saline (PBS). The controlled release of MTX / LDHs nanocomposites was investigated and their kinetics were fitted. The results showed that MTX / LDHs nanocomposites The release process of the complex was controlled by Fick diffusion-controlled ion exchange and intragranular diffusion process.Finally MTT assay was used to investigate the inhibitory effect of MTX / LDHs nanocomposites on the proliferation of lung cancer A549 cells. The results showed that the pure MTX With better inhibition of cancer cell proliferation.