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现在大剂量化疗合并自身外周血干细胞解救已被广泛应用,为了简化这项技术,作者采用了大容量单采外周血干细胞(BSC)及非程控-80℃冷冻保存的方法。研究对象包括多发性骨髓瘤(MM)8例,非霍奇金淋巴瘤(NHL)7例,霍奇金病(HK)2例,急性髓细胞白血病(AML)第三次完全缓解1例,高危急性淋巴细胞白血病(ALL)第1次完全缓解1例,无性细胞瘤(DYE)4例,乳腺癌(BRE)3例。用化疗+粒细胞系集落刺激因子(G-CSF)或单独使用G-CSF后第4天或第5天,当WBC>1×10~9/L并且外周血CD34~+细胞>0.5％时,收集BSC,在COBE Spectra细胞分离机上进行大容量
Now that high-dose chemotherapy combined with the rescue of its peripheral blood stem cells has been widely used, in order to simplify this technique, the authors used a large-capacity peripheral blood stem cell (BSC) and non-programmed -80 °C cryopreservation method. The subjects included 8 cases of multiple myeloma (MM), 7 cases of non-Hodgkin’s lymphoma (NHL), 2 cases of Hodgkin’s disease (HK), and 1 case of third acute complete myeloid leukemia (AML) remission. The first complete remission of high-risk acute lymphoblastic leukemia (ALL) occurred in 1 patient, dysplasia (DYE) in 4 patients, and breast cancer (BRE) in 3 patients. On day 4 or day 5 after chemotherapy + granulocyte cell colony-stimulating factor (G-CSF) or G-CSF alone, when WBC> 1 × 10-9/L and peripheral blood CD34 + cells> 0.5% , Collect BSC, Perform Large Capacity on COBE Spectra Cell Separator