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目的:探讨青龙木叶皂苷的镇痛效应及其机制。方法:60只Wistar大鼠随机分为5组(每组12只):青龙木叶皂苷15、30、60μg.kg-1组,青龙木叶皂苷加纳洛酮组及生理盐水对照组。通过预先手术埋置的脑室套管向侧脑室注射青龙木叶皂苷,用辐射热甩尾法测定大鼠痛阈。结果:侧脑室注射3种不同剂量青龙木叶皂苷组的痛阈在注射5 min后与生理盐水对照组比较有不同程度的提高。15μg.kg-1组的痛阈虽然有提高,但与对照组比较差异无显著性(P>0.05);30μg.kg-1组的痛阈提高持续50 min,60μg.kg-1组的痛阈提高可持续60 min以上,与对照组比较差异有显著性(P<0.05或P<0.01)。对照组大鼠的痛阈在60 min内无明显变化(P>0.05)。且青龙木叶皂苷镇痛效应可被纳洛酮所翻转。结论:青龙木叶皂苷具有较强镇痛效应及剂量-镇痛效应关系,其镇痛作用(痛阈升高)是通过脑内μ阿片受体实现的。
Objective: To investigate the analgesic effect and mechanism of saponin from Qinglong. METHODS: Sixty Wistar rats were randomly divided into 5 groups (12 in each group): Qinglong xanthosaponin 15, 30, 60 μg.kg-1 group, Qinglong xanthosaponin plus naloxone group and normal saline control group. Intravenous saponin was injected into the lateral ventricle through a preoperatively implanted ventricular socket, and the rat pain threshold was measured using a radiant heat flick method. Results: The pain threshold of three different doses of Qinglong silyridin group injected into the lateral ventricle was increased by 5 min after injection compared with the saline control group. Although the pain threshold was increased in the 15μg.kg-1 group, there was no significant difference from the control group (P>0.05); the pain threshold in the 30μg.kg-1 group increased for 50 min, and the pain in the 60μg.kg-1 group was not significant. The threshold increase was more than 60 min, and there was significant difference compared with the control group (P<0.05 or P<0.01). The pain threshold of the control group rats did not change significantly within 60 minutes (P>0.05). In addition, the analgesic effect of saponin from Qinglong can be reversed by naloxone. Conclusion: Qinglong saponin has a strong analgesic effect and dose-analgesic effect, and its analgesic effect (increased pain threshold) is achieved by intracerebral μ opioid receptors.