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采用淹水非种植水稻土微环境模式系统,对水稻土进行1 h和1、5、10、20、30 d淹水培养,利用PCR-DGGE技术检测、多元统计分析淹水培养过程中芽孢杆菌的群落结构和多样性变化规律及其影响因子。结果表明,淹水培养过程中芽孢杆菌群落结构发生了明显的演替性变化:淹水培养前期(1 h~5 d)R-策略芽孢杆菌占优势,并演替至中后期(5~30 d);后期(10~30 d)K-对策芽孢杆菌占优势且逐渐趋于平衡。在培养初期(1 h~1 d),芽孢杆菌属微生物优势种群相似度较高且多样性指数变化较小;1~5 d期间多样性指数有较大变化;在培养后期(20~30 d),优势种群相似度最高且多样性指数趋于平衡。DCCA分析将淹水处理过程分成早期、中期和末期3类不同的生境,CCA分析进一步显示淹水过程中水稻土二价铁含量及芽孢杆菌的多样性指数H’、Ds和丰富度指数dMa变化与上述生境产生有相关性。测序结果表明,除HZ-B1外,其余9个优势DGGE条带均属于芽孢杆菌属细菌,且大多与来自不同地域的水稻土的芽孢杆菌关系密切。
The paddy soil was flooded at 1 h, 1, 5, 10, 20, 30 d after flooding and non-planting paddy soil micro-environment model. PCR-DGGE was used to detect paddy soil. Multivariate statistical analysis was performed on the Bacillus subtilis The community structure and diversity of the law of variation and its impact factors. The results showed that the Bacillus subtilis community structure changed evidently in the process of flooding: R- strategy Bacillus was predominant in the early stage of flooding (1 h ~ 5 d) d). In the later stage (10 ~ 30 d), K-strategy Bacillus dominated and gradually became more balanced. In the early stage of culture (1 h ~ 1 d), the dominant species of Bacillus sp. Had higher similarity and less diversity index, while the diversity index varied greatly from 1 to 5 days. In the later period of cultivation (20-30 days ), The highest similarity of dominant populations and the diversity index tends to balance. DCCA analysis divided the flooding process into three different habitats in the early, middle and late stages. CCA analysis further showed that the ferrous iron content of paddy soil and Bacillus diversity index H ’, Ds and abundance index dMa in flooding Relevant to the above habitat. Sequencing results showed that except for HZ-B1, the other nine dominant DGGE bands belonged to Bacillus sp., Most of which were closely related to Bacillus from paddy soils in different regions.